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Literature summary extracted from

  • Paoletti, F.; Aldinucci, D.
    Immunoaffinity purification of rat liver transketolase: evidence for multiple forms of the enzyme (1986), Arch. Biochem. Biophys., 245, 212-219.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.2.1.1 glycerol, 10% v/v, stabilizes Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.1 Rabbit antibodies not Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.1 0.44
-
 D-xylulose 5-phosphate (+ D-ribose 5-phosphate) Rattus norvegicus
2.2.1.1 0.72
-
 D-ribose 5-phosphate cosubstrate D-xylulose 5-phosphate Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.2.1.1 cytosol
-
 Rattus norvegicus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.2.1.1 69000
-
 alpha2, 2 * 69000, SDS-PAGE Rattus norvegicus
2.2.1.1 139000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate Rattus norvegicus
-
 sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.1 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.1 immunoaffinity chromatography Rattus norvegicus
2.2.1.1 partial, 5 multiple forms by isoelectric focusing Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.2.1.1 liver
-
 Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
2.2.1.1 -20°C, more than a week Rattus norvegicus
2.2.1.1 -20°C, up to 2 months with 10% v/v glycerol Rattus norvegicus
2.2.1.1 0-4°C, several h Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate
-
 Rattus norvegicus sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.2.1.1 dimer alpha2, 2 * 69000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.2.1.1 25
-
 assay at Rattus norvegicus
2.2.1.1 45
-
 assay at, low enzyme concentration Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2.1.1 8
-
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.2.1.1 6.5 10.2 about half-maximal activity at pH 6.5 and 10.2 Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.2.1.1 6.5
-
 below, unstable Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.1 thiamine diphosphate 2 mol thiamine diphosphate per mol enzyme Rattus norvegicus