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Literature summary extracted from

  • Kochetov, G.A.
    Transketolase from yeast, rat liver, and pig liver (1982), Methods Enzymol., 90, 209-223.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.2.1.1
-
 Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.1 Oxythiamine diphosphate
-
 Saccharomyces cerevisiae
2.2.1.1 phosphate
-
 Saccharomyces cerevisiae
2.2.1.1 sulfate
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.1 0.21
-
 D-xylulose 5-phosphate
-
 Saccharomyces cerevisiae
2.2.1.1 0.4
-
 D-ribose 5-phosphate
-
 Saccharomyces cerevisiae
2.2.1.1 1.8
-
 fructose 6-phosphate
-
 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.2.1.1 cytosol
-
 Rattus norvegicus 5829
-
2.2.1.1 cytosol
-
 Sus scrofa 5829
-
2.2.1.1 cytosol
-
 Saccharomyces cerevisiae 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.1 Mg2+ requirement Rattus norvegicus
2.2.1.1 Mg2+ requirement Sus scrofa
2.2.1.1 Mg2+ requirement Saccharomyces cerevisiae
2.2.1.1 Mg2+ no exogenous Mg2+-addition necessary Rattus norvegicus
2.2.1.1 Mg2+ no exogenous Mg2+-addition necessary Sus scrofa
2.2.1.1 Mg2+ no exogenous Mg2+-addition necessary Saccharomyces cerevisiae
2.2.1.1 Mg2+ indispensable for thiamine diphosphate/enzyme binding Rattus norvegicus
2.2.1.1 Mg2+ indispensable for thiamine diphosphate/enzyme binding Sus scrofa
2.2.1.1 Mg2+ indispensable for thiamine diphosphate/enzyme binding Saccharomyces cerevisiae
2.2.1.1 additional information
-
 Rattus norvegicus
2.2.1.1 additional information
-
 Saccharomyces cerevisiae
2.2.1.1 additional information no addition of exogenous metal ions required Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.2.1.1 130000
-
 sedimentation equilibrium method Rattus norvegicus
2.2.1.1 138000
-
 meniscus depletion method Sus scrofa
2.2.1.1 158000 159000 sedimentation equilibrium data Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate Rattus norvegicus
-
 sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate Sus scrofa
-
 sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate Saccharomyces cerevisiae
-
 sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.1 Rattus norvegicus
-
-
-
2.2.1.1 Saccharomyces cerevisiae
-
 baker's yeast
-
2.2.1.1 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.1
-
 Rattus norvegicus
2.2.1.1
-
 Sus scrofa
2.2.1.1
-
 Saccharomyces cerevisiae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.2.1.1 liver
-
 Rattus norvegicus
-
2.2.1.1 liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2.1.1 0.88
-
-
 Sus scrofa
2.2.1.1 1.5
-
-
 Rattus norvegicus
2.2.1.1 20
-
-
 Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
2.2.1.1 -20°C, several days Sus scrofa
2.2.1.1 4°C, several weeks in 8 M glycylglycine buffer, pH 7.4 Saccharomyces cerevisiae
2.2.1.1 frozen, at least a week Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate
-
 Rattus norvegicus sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate
-
 Sus scrofa sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate
-
 Saccharomyces cerevisiae sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate L-erythrulose Rattus norvegicus sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate L-erythrulose Sus scrofa sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate L-erythrulose Saccharomyces cerevisiae sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate glycolaldehyde Rattus norvegicus sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate glycolaldehyde Sus scrofa sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate glycolaldehyde Saccharomyces cerevisiae sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate and acceptor substrates may be: D-glyceraldehyde 3-phosphate Rattus norvegicus sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate and acceptor substrates may be: D-glyceraldehyde 3-phosphate Sus scrofa sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate and acceptor substrates may be: D-glyceraldehyde 3-phosphate Saccharomyces cerevisiae sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate D-ribose 5-phosphate Rattus norvegicus sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate D-ribose 5-phosphate Sus scrofa sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate D-ribose 5-phosphate Saccharomyces cerevisiae sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate D-erythrose 4-phosphate Rattus norvegicus sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate D-erythrose 4-phosphate Sus scrofa sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate D-erythrose 4-phosphate Saccharomyces cerevisiae sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate sedoheptulose 7-phosphate Rattus norvegicus sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate sedoheptulose 7-phosphate Sus scrofa sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate sedoheptulose 7-phosphate Saccharomyces cerevisiae sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate wide specificity for both reactants: donor substrates may be: fructose 6-phosphate Rattus norvegicus sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate wide specificity for both reactants: donor substrates may be: fructose 6-phosphate Sus scrofa sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate wide specificity for both reactants: donor substrates may be: fructose 6-phosphate Saccharomyces cerevisiae sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r
2.2.1.1 hydroxypyruvate + D-glyceraldehyde 3-phosphate
-
 Rattus norvegicus CO2 + ribulose 5-phosphate
-
 ir
2.2.1.1 hydroxypyruvate + D-glyceraldehyde 3-phosphate
-
 Sus scrofa CO2 + ribulose 5-phosphate
-
 ir
2.2.1.1 hydroxypyruvate + D-glyceraldehyde 3-phosphate
-
 Saccharomyces cerevisiae CO2 + ribulose 5-phosphate
-
 ir

Subunits

EC Number Subunits Comment Organism
2.2.1.1 More at sufficiently low concentration the apo-, not the holoenzyme dissociates reversibly into 2 subunits of equal molecular weight, individual subunits are equally catalytically active Saccharomyces cerevisiae
2.2.1.1 tetramer alpha2beta2, 2 * 27000-29000 + 2 * 52000-56000, SDS-PAGE Sus scrofa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.2.1.1 additional information
-
 thiamine diphosphate enhances thermal stability Sus scrofa
2.2.1.1 20
-
 at pH 6.5-8.7, 20 min stable Sus scrofa
2.2.1.1 40
-
 pH 8.0, activity hardly changes for 60 min Sus scrofa
2.2.1.1 50
-
 t1/2: 10 min Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2.1.1 additional information
-
-
 Rattus norvegicus
2.2.1.1 additional information
-
-
 Saccharomyces cerevisiae
2.2.1.1 additional information
-
 pI: 7.6-7.8 Sus scrofa
2.2.1.1 7.6
-
-
 Rattus norvegicus
2.2.1.1 7.6
-
-
 Sus scrofa
2.2.1.1 7.6
-
-
 Saccharomyces cerevisiae
2.2.1.1 7.8 8.2
-
 Sus scrofa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.2.1.1 5
-
 thiamine diphosphate dissociates from holoenzyme Sus scrofa
2.2.1.1 6.5 8.7 20°C, 20 min stable Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.1 thiamine diphosphate with different affinities for the cofactor Rattus norvegicus
2.2.1.1 thiamine diphosphate with different affinities for the cofactor Sus scrofa
2.2.1.1 thiamine diphosphate with different affinities for the cofactor Saccharomyces cerevisiae
2.2.1.1 thiamine diphosphate 2 mol thiamine diphosphate per mol enzyme Rattus norvegicus
2.2.1.1 thiamine diphosphate 2 mol thiamine diphosphate per mol enzyme Sus scrofa
2.2.1.1 thiamine diphosphate 2 mol thiamine diphosphate per mol enzyme Saccharomyces cerevisiae