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Literature summary extracted from

  • Szilagyi, I.; Vargha, G.; Szabo, G.
    Ornithine carbamoyltransferase of Streptomyces fradiae: purification and properties (1987), FEMS Microbiol. Lett., 48(1), 115-119.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.3.3 L-2-aminobutyrate 5-20 mM; L-isomer Streptomyces fradiae
2.1.3.3 ornithine above 8 mM Streptomyces fradiae
2.1.3.3 Zn2+ 5-20 mM Streptomyces fradiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.3.3 40000
-
 x * 40000, enzyme may be a dimer, SDS-PAGE Streptomyces fradiae
2.1.3.3 87000
-
 gel filtration Streptomyces fradiae

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.3 Streptomyces fradiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.3 ammonium sulfate, DEAE-cellulose, aminohexyl-agarose Streptomyces fradiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.3.3 50
-
-
 Streptomyces fradiae
2.1.3.3 500
-
-
 Streptomyces fradiae

Storage Stability

EC Number Storage Stability Organism
2.1.3.3 0-4°C, 2 months, no loss of activity Streptomyces fradiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.3 carbamoyl phosphate + L-ornithine
-
 Streptomyces fradiae phosphate + L-citrulline
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.3.3 ? x * 40000, enzyme may be a dimer, SDS-PAGE Streptomyces fradiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.3.3 40 45
-
 Streptomyces fradiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.3.3 9.5 10
-
 Streptomyces fradiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.3.3 7 10 sharp decrease in activity below pH 9.0 Streptomyces fradiae