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Literature summary extracted from

  • Legrain, C.; Stalon, V.
    Ornithine carbamoyltransferase from Escherichia coli W. Purification, structure and steady-state kinetic analysis (1976), Eur. J. Biochem., 63, 289-301.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.3.3 2,4-diaminobutyrate
-
 Escherichia coli
2.1.3.3 arsenate competitive vs. carbamoylphosphate, noncompetitive vs. ornithine Escherichia coli
2.1.3.3 glycine
-
 Escherichia coli
2.1.3.3 L-2,4-diaminobutyric acid
-
 Escherichia coli
2.1.3.3 L-alanine
-
 Escherichia coli
2.1.3.3 L-asparagine weak Escherichia coli
2.1.3.3 L-isoleucine
-
 Escherichia coli
2.1.3.3 L-leucine
-
 Escherichia coli
2.1.3.3 L-lysine weak Escherichia coli
2.1.3.3 L-norvaline
-
 Escherichia coli
2.1.3.3 L-serine weak Escherichia coli
2.1.3.3 L-valine
-
 Escherichia coli
2.1.3.3 methionine L-isomer Escherichia coli
2.1.3.3 ornithine at high concentrations; noncompetitive substrate inhibition vs. carbamoylphosphate Escherichia coli
2.1.3.3 phosphate competitive vs. carbamoylphosphate, noncompetitive vs. ornithine Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.3.3 35000
-
 3 * 35000, SDS-PAGE Escherichia coli
2.1.3.3 105000
-
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.3.3 carbamoyl phosphate + L-ornithine Escherichia coli enzyme belongs to the arginine biosynthesis pathway phosphate + L-citrulline
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.3 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.3 heat, ammonium sulfate, DEAE-Sephadex, Sephadex G-200, aminohexyl-Sepharose Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.3.3 carbamoyl phosphate + L-ornithine = phosphate + L-citrulline mechanism Escherichia coli
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.3.3 2833
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.3 carbamoyl phosphate + L-ornithine
-
 Escherichia coli phosphate + L-citrulline
-
 r
2.1.3.3 carbamoyl phosphate + L-ornithine enzyme belongs to the arginine biosynthesis pathway Escherichia coli phosphate + L-citrulline
-
 r

Subunits

EC Number Subunits Comment Organism
2.1.3.3 trimer 3 * 35000, SDS-PAGE Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.3.3 68
-
 half-life: 5 min, 8 min with 500 mM KCl, 10 min with 10 mM citrulline, 20 min with 100 mM citrulline, 43 min with 20 mM phosphate, 48 min with 100 mM ornithine, 108 min with 100 mM phosphate, 120 min with 10 mM phosphate and 10 mM ornithine, 122 min with 10 mM phosphate and 100 mM citrulline, more than 200 min with 100 mM phosphate and 100 mM citrulline or with 10 mM phosphate and 100 mM ornithine Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.3.3 0.4
-
 L-norvaline
-
 Escherichia coli
2.1.3.3 5.5
-
 L-leucine
-
 Escherichia coli
2.1.3.3 7
-
 2,4-diaminobutyrate
-
 Escherichia coli
2.1.3.3 13
-
 L-alanine
-
 Escherichia coli
2.1.3.3 13.5
-
 L-valine
-
 Escherichia coli
2.1.3.3 14
-
 L-isoleucine
-
 Escherichia coli
2.1.3.3 15
-
 glycine
-
 Escherichia coli
2.1.3.3 40
-
 L-methionine
-
 Escherichia coli
2.1.3.3 63.5
-
 L-serine
-
 Escherichia coli
2.1.3.3 110
-
 L-asparagine
-
 Escherichia coli