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Literature summary extracted from

  • Hack, E.S.; Vorobyova, T.; Sakash, J.B.; West, J.M.; Macol, C.P.; Herve, G.; Williams, M.K.; Kantrowitz, E.R.
    Characterization of the aspartate transcarbamoylase from Methanococcus jannaschii (2000), J. Biol. Chem., 275, 15820-15827.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.3.2
-
 Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.3.2 10.9
-
 L-aspartate at 55°C Methanocaldococcus jannaschii
2.1.3.2 18.2
-
 L-aspartate at 37°C Methanocaldococcus jannaschii
2.1.3.2 34.6
-
 L-aspartate at 25°C Methanocaldococcus jannaschii
2.1.3.2 40.4
-
 L-aspartate at 15°C Methanocaldococcus jannaschii
2.1.3.2 88
-
 Carbamoyl phosphate at 25°C Methanocaldococcus jannaschii
2.1.3.2 162
-
 Carbamoylphosphate at 37°C Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.3.2 17000
-
 x * 37000 + x * 17000, most probably a dodecamer, SDS-PAGE Methanocaldococcus jannaschii
2.1.3.2 32000
-
 regulatory subunit, gel filtration Methanocaldococcus jannaschii
2.1.3.2 37000
-
 x * 37000 + x * 17000, most probably a dodecamer, SDS-PAGE Methanocaldococcus jannaschii
2.1.3.2 129000
-
 catalytic subunit, gelfiltration Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.3.2 carbamoylphosphate + L-aspartate Escherichia coli
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate Methanocaldococcus jannaschii
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.2 Escherichia coli
-
-
-
2.1.3.2 Methanocaldococcus jannaschii Q58976
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.2 heat, Q-Sepharose, Phenyl-Sepharose Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.2 carbamoyl phosphate + L-aspartate
-
 Escherichia coli phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoyl phosphate + L-aspartate
-
 Methanocaldococcus jannaschii phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate
-
 Escherichia coli phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate
-
 Methanocaldococcus jannaschii phosphate + N-carbamoyl-L-aspartate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.3.2 ? x * 37000 + x * 17000, most probably a dodecamer, SDS-PAGE Methanocaldococcus jannaschii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.3.2 75
-
 catalytic subunit looses 25% activity after 60 min Methanocaldococcus jannaschii
2.1.3.2 75
-
 catalytic subunit looses 50% activity after 1 min Escherichia coli