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Literature summary extracted from

  • Masood, R.; Venkitasubramanian, T.A.
    Purification and properties of aspartate transcarbamylase from Mycobacterium smegmatis (1988), Biochim. Biophys. Acta, 953, 106-113.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.1.3.2 carbamoyl phosphate protects against thermal denaturation Mycolicibacterium smegmatis
2.1.3.2 freezing and thawing in 50 mM Tris-HCl buffer, pH 7.4, 1 mM 2-mercaptoethanol has no effect on stability Mycolicibacterium smegmatis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.3.2 Ca2+
-
Mycolicibacterium smegmatis
2.1.3.2 HgSO4
-
Mycolicibacterium smegmatis
2.1.3.2 iodoacetate
-
Mycolicibacterium smegmatis
2.1.3.2 Maleate
-
Mycolicibacterium smegmatis
2.1.3.2 Mn2+
-
Mycolicibacterium smegmatis
2.1.3.2 Ni2+
-
Mycolicibacterium smegmatis
2.1.3.2 p-hydroxymercuribenzoate
-
Mycolicibacterium smegmatis
2.1.3.2 phosphate
-
Mycolicibacterium smegmatis
2.1.3.2 succinate
-
Mycolicibacterium smegmatis
2.1.3.2 UMP
-
Mycolicibacterium smegmatis
2.1.3.2 Zn2+
-
Mycolicibacterium smegmatis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.3.2 additional information no requirement for any metal ion Mycolicibacterium smegmatis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.3.2 108000
-
1 * 138000 + 1 * 108000, SDS-PAGE Mycolicibacterium smegmatis
2.1.3.2 138000
-
1 * 138000 + 1 * 108000, SDS-PAGE Mycolicibacterium smegmatis
2.1.3.2 246000
-
gel filtration Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.3.2 carbamoylphosphate + L-aspartate Mycolicibacterium smegmatis
-
phosphate + N-carbamoyl-L-aspartate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.2 Mycolicibacterium smegmatis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.2 streptomycin sulfate, ammonium sulfate, DE-52 column, Sephadex G-200, aspartate linked CNBr Mycolicibacterium smegmatis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.3.2 7900
-
-
Mycolicibacterium smegmatis

Storage Stability

EC Number Storage Stability Organism
2.1.3.2 -20°C, 2 mM carbamoyl phosphate, 1 mM 2-mercaptoethanol, at least a week, no loss of activity Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.2 carbamoyl phosphate + L-aspartate
-
Mycolicibacterium smegmatis phosphate + N-carbamoyl-L-aspartate
-
?
2.1.3.2 carbamoylphosphate + L-aspartate
-
Mycolicibacterium smegmatis phosphate + N-carbamoyl-L-aspartate
-
?

Subunits

EC Number Subunits Comment Organism
2.1.3.2 dimer 1 * 138000 + 1 * 108000, SDS-PAGE Mycolicibacterium smegmatis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.3.2 45
-
loss of activity after 10 min, carbamoylphosphate protects Mycolicibacterium smegmatis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.3.2 7.8
-
-
Mycolicibacterium smegmatis