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Literature summary extracted from

  • Shenoy, B.C.; Xie, Y.; Sha, D.; Samols, D.
    Identification and characterization of a factor which is essential for assembly of transcarboxylase (1993), Biochemistry, 32, 10750-10756.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.3.1 1.3S subunit cloned and expressed in Escherichia coli Propionibacterium freudenreichii subsp. shermanii
2.1.3.1 5S subunit cloned and expressed in Escherichia coli Propionibacterium freudenreichii subsp. shermanii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.3.1
-
 Propionibacterium freudenreichii subsp. shermanii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.3.1 additional information loss of assembly-promoting factor activity after digestion with trypsin, V8 protease, pronase Propionibacterium freudenreichii subsp. shermanii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.3.1 120000
-
 the 26S holoenzyme consists of a hexameric central 12S subunit, 360000 Da and six peripheral dimeric 5S subunits, 6 * 120000 Da each of which is linked to the central subunit by 2 biotinyl 1.3S subunits, 12 * MW 12000 Propionibacterium freudenreichii subsp. shermanii

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.1 Propionibacterium freudenreichii subsp. shermanii
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.3.1 additional information
-
-
 Propionibacterium freudenreichii subsp. shermanii
2.1.3.1 2.3
-
-
 Propionibacterium freudenreichii subsp. shermanii
2.1.3.1 43
-
 recombinant 5S subunit + assembly-promotin factor, overall reaction Propionibacterium freudenreichii subsp. shermanii
2.1.3.1 48
-
-
 Propionibacterium freudenreichii subsp. shermanii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.1 propionyl-CoA + oxaloacetate two partial reactions Propionibacterium freudenreichii subsp. shermanii (S)-methylmalonyl-CoA + pyruvate
-
 r

Subunits

EC Number Subunits Comment Organism
2.1.3.1 More existence of a novel, previously unidentified subunit, a assemply-promoting factor, APF, which is nessessary for the assemply of enzyme, MW: 60000 Da, SDS-PAGE Propionibacterium freudenreichii subsp. shermanii
2.1.3.1 More the 26S holoenzyme consists of a hexameric central 12S subunit, 360000 Da and six peripheral dimeric 5S subunits, 6 * 120000 Da each of which is linked to the central subunit by 2 biotinyl 1.3S subunits, 12 * MW 12000 Propionibacterium freudenreichii subsp. shermanii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.3.1 additional information
-
 assembly-promoting factor heat stable Propionibacterium freudenreichii subsp. shermanii