Literature summary extracted from
Woo, S.B.; Shenoy, B.C.; Wood, H.G.; Magner, W.J.; Kumar, G.K.; Beegen, H.; Samols, D.
Effect of deletion from the carboxyl terminus of the 12 S subunit on activity of transcarboxylase (1993), J. Biol. Chem., 268, 16413-16419.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.1.3.1 |
12S subunit cloned and expressed in Escherichia coli |
Propionibacterium freudenreichii subsp. shermanii |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
2.1.3.1 |
120000 |
- |
the 26S holoenzyme consists of a hexameric central 12S subunit, 360000 Da and six peripheral dimeric 5S subunits, 6 * 120000 Da each of which is linked to the central subunit by 2 biotinyl 1.3S subunits, 12 * MW 12000 |
Propionibacterium freudenreichii subsp. shermanii |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.1.3.1 |
Propionibacterium freudenreichii subsp. shermanii |
- |
- |
- |
Posttranslational Modification
EC Number |
Posttranslational Modification |
Comment |
Organism |
---|
2.1.3.1 |
side-chain modification |
97 amino acids deleted of the carboxyl-terminal region of the monomer of the 12S subunit, 46% loss of activity because of a reduces stability of the 12S subunit and a decrease in binding of 6S subunit to the 12 subunit |
Propionibacterium freudenreichii subsp. shermanii |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
2.1.3.1 |
(S)-methylmalonyl-CoA + pyruvate = propanoyl-CoA + oxaloacetate |
partial reaction 1 is catalysed specifically by the 12S subunit, partial reaction 2 is catalysed specifically by the 5S subunit |
Propionibacterium freudenreichii subsp. shermanii |
|
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
2.1.3.1 |
additional information |
- |
comparison of wild-type and truncated forms |
Propionibacterium freudenreichii subsp. shermanii |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.1.3.1 |
propionyl-CoA + oxaloacetate |
two partial reactions |
Propionibacterium freudenreichii subsp. shermanii |
(S)-methylmalonyl-CoA + pyruvate |
- |
r |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.1.3.1 |
More |
the 26S holoenzyme consists of a hexameric central 12S subunit, 360000 Da and six peripheral dimeric 5S subunits, 6 * 120000 Da each of which is linked to the central subunit by 2 biotinyl 1.3S subunits, 12 * MW 12000 |
Propionibacterium freudenreichii subsp. shermanii |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
2.1.3.1 |
biotin |
requirement |
Propionibacterium freudenreichii subsp. shermanii |
|