Literature summary extracted from
Shenoy, B.C.; Kumar, G.K.; Samols, D.
Dissection of the biotinyl subunit of transcarboxylase into regions essential for activity and assembly (1993), J. Biol. Chem., 268, 2232-2238.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.1.3.1 |
1.3S subunit cloned and expressed in Escherichia coli |
Propionibacterium freudenreichii subsp. shermanii |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.1.3.1 |
Propionibacterium freudenreichii subsp. shermanii |
- |
- |
- |
Posttranslational Modification
EC Number |
Posttranslational Modification |
Comment |
Organism |
---|
2.1.3.1 |
side-chain modification |
a series of truncated forms of the 1.3S subunit is prepared and the effect on the first and the second partial reaction and the overall enzyme reaction measured, result: the sequence 59-78 of 1.3S subunit is necessary for the carboxylation of biotin of the 1.3 S subunit, the inability of the truncated peptides to form enzyme complexes explains the activity loss in the overall reaction |
Propionibacterium freudenreichii subsp. shermanii |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.1.3.1 |
recombinant enzyme and mutant 1.3S subunit |
Propionibacterium freudenreichii subsp. shermanii |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
2.1.3.1 |
(S)-methylmalonyl-CoA + pyruvate = propanoyl-CoA + oxaloacetate |
mechanism |
Propionibacterium freudenreichii subsp. shermanii |
|
2.1.3.1 |
(S)-methylmalonyl-CoA + pyruvate = propanoyl-CoA + oxaloacetate |
partial reaction 1 is catalysed specifically by the 12S subunit, partial reaction 2 is catalysed specifically by the 5S subunit |
Propionibacterium freudenreichii subsp. shermanii |
|
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.1.3.1 |
propionyl-CoA + oxaloacetate |
two partial reactions |
Propionibacterium freudenreichii subsp. shermanii |
(S)-methylmalonyl-CoA + pyruvate |
- |
r |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.1.3.1 |
More |
5S subunit is a dimer of identical monomers, contains Co2+ and Zn2+ and assembles with two 1.3 subunits to form a stable complex termed the 6S subunit |
Propionibacterium freudenreichii subsp. shermanii |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
2.1.3.1 |
biotin |
requirement |
Propionibacterium freudenreichii subsp. shermanii |
|