Literature summary extracted from

Gambini, A.; Crosti, P.; Bianchetti, R.
Inhibitor studies of methionyl-tRNA transformylase of Euglena gracilis (1980), Biochim. Biophys. Acta, 613, 73-78.

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
2.1.2.9	10-Formylaminopterin	0.01 mM: 38% inhibition, 0.1 mM: 62% inhibition, competitive	Euglena gracilis
2.1.2.9	10-formylpteroylglutamic acid	0.1 mM: 31% inhibition, 1 mM: 52% inhibition, competitive	Euglena gracilis
2.1.2.9	5,10-methylenetetrahydrofolate	-	Euglena gracilis
2.1.2.9	5-formyltetrahydrofolate	competitive inhibition	Euglena gracilis
2.1.2.9	5-methyltetrahydrofolate	most effective, non-competitive inhibitor	Euglena gracilis
2.1.2.9	aminopterin	1 mM: 26% inhibition, competitive inhibition	Euglena gracilis
2.1.2.9	methotrexate	0.01 mM: 30% inhibition, 0.1 mM, 52% inhibition, competitive	Euglena gracilis
2.1.2.9	additional information	inhibition studies, not inhibited by trimethoprim; not inhibited by folic acid	Euglena gracilis
2.1.2.9	reduced aminopterin	1 mM, 34% inhibition, competitive inhibition	Euglena gracilis
2.1.2.9	tetrahydrofolate	competitive, multisite inhibition	Euglena gracilis

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
2.1.2.9	chloroplast	-	Euglena gracilis	9507	-

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.1.2.9	Euglena gracilis	-	-	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.1.2.9	10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O	high specificity for initiator tRNAMet, strong positive cooperativity for Met-tRNAfMet and N10-formyltetrahydrofolate	Euglena gracilis	tetrahydrofolate + N-formylmethionyl-tRNAfMet	-	?

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
2.1.2.9	10-formyltetrahydrofolate	-	Euglena gracilis
2.1.2.9	tetrahydrofolate	-	Euglena gracilis

Ki Value [mM]

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
2.1.2.9	0.0002	-	tetrahydrofolate	-	Euglena gracilis
2.1.2.9	0.003	-	5-methyltetrahydrofolate	-	Euglena gracilis
2.1.2.9	0.045	-	5-formyltetrahydrofolate	-	Euglena gracilis

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Release 2023.1 (January 2023)