Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Subramaniam, R.; Wang, Y.; Mathews, C.K.; Santi, D.V.
    On the inhibition of deoxycytidylate hydroxymethylase by 5-fluoro-2-deoxycytidine 5-monophosphate (1989), Arch. Biochem. Biophys., 275, 11-15.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.2.8 5-Fluoro-2'-deoxycytidine 5'-monophosphate competitive inhibitor with respect to dCMP, not mechanism-based inhibitor Tequatrovirus T4

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.8 0.36
-
 deoxycytidylate
-
 Tequatrovirus T4

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.8 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate Tequatrovirus T4
-
 tetrahydrofolate + 5-hydroxymethyldeoxycytidylate enzyme is responsible for production of 5-hydroxymethyl-dCMP found in the DNA of the phage ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.8 Tequatrovirus T4
-
 Escherichia coli infected with bacteriophage T4
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.8
-
 Tequatrovirus T4

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.2.8 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate = tetrahydrofolate + 5-hydroxymethyldeoxycytidylate mechanism Tequatrovirus T4
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.8 1
-
-
 Tequatrovirus T4

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.8 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
-
 Tequatrovirus T4 tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
-
 ?
2.1.2.8 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
-
 Tequatrovirus T4 tetrahydrofolate + 5-hydroxymethyldeoxycytidylate enzyme is responsible for production of 5-hydroxymethyl-dCMP found in the DNA of the phage ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.2.8 25
-
 assay at Tequatrovirus T4

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.8 5,10-methylenetetrahydrofolate as cofactor Tequatrovirus T4
2.1.2.8 tetrahydrofolate
-
 Tequatrovirus T4

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.2.8 0.025
-
 5-Fluoro-2'-deoxycytidine 5'-monophosphate
-
 Tequatrovirus T4