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Literature summary extracted from

  • Wilson Miles, E.
    alpha-Methylserine transhydroxymethylase (Pseudomonas) (1971), Methods Enzymol., 17B, 341-346.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.2.7
-
 Pseudomonas sp.

General Stability

EC Number General Stability Organism
2.1.2.7 prolonged dialysis inactivates Pseudomonas sp.
2.1.2.7 pyridoxal 5-phosphate restores activity Pseudomonas sp.
2.1.2.7 pyridoxal 5-phosphate stabilizes during purification Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.2.7 Cu2+ at higher concentrations Pseudomonas sp.
2.1.2.7 DL-cycloserine strong, competitive Pseudomonas sp.
2.1.2.7 Fe2+ at higher concentrations Pseudomonas sp.
2.1.2.7 Fe3+ at higher concentrations Pseudomonas sp.
2.1.2.7 L-Cycloserine
-
 Pseudomonas sp.
2.1.2.7 L-serine weak, competitive Pseudomonas sp.
2.1.2.7 additional information no inhibition by Mg2+, Mn2+, Al3+, 2-mercaptoethanol Pseudomonas sp.
2.1.2.7 p-chloromercuribenzoate
-
 Pseudomonas sp.
2.1.2.7 Zn2+ at higher concentrations Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.7 0.25
-
 L-tetrahydrofolate pH 7.5, calculated on the basis of one isomer Pseudomonas sp.
2.1.2.7 0.8
-
 hydroxymethylserine pH 7.5 Pseudomonas sp.
2.1.2.7 1.5
-
 2-methyl-DL-serine pH 7.5 Pseudomonas sp.
2.1.2.7 6.2
-
 2-ethyl-DL-serine pH 7.5 Pseudomonas sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.2.7 cytoplasm
-
 Pseudomonas sp. 5737
-

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.7 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.7
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.7 11.1
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.7 tetrahydrofolate + (+)-2-methylserine stereospecific, dimedon cannot replace tetrahydrofolate Pseudomonas sp. 5,10-methylenetetrahydrofolate + D-alanine + H2O
-
 r
2.1.2.7 tetrahydrofolate + (-)-ethylserine
-
 Pseudomonas sp. 5,10-methylenetetrahydrofolate + D-2-aminobutyrate + H2O
-
 r
2.1.2.7 tetrahydrofolate + 2-ethyl-DL-serine
-
 Pseudomonas sp. 5,10-methylenetetrahydrofolate + D-2-aminobutyrate + H2O
-
 r
2.1.2.7 tetrahydrofolate + 2-hydroxymethylserine specific removal of one of the two hydroxyl groups Pseudomonas sp. 5,10-methylenetetrahydrofolate + D-serine + H2O
-
 r
2.1.2.7 tetrahydrofolate + 2-methyl-DL-serine
-
 Pseudomonas sp. 5,10-methylenetetrahydrofolate + D-alanine + H2O
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.2.7 45
-
-
 Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.2.7 9
-
-
 Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.7 pyridoxal 5'-phosphate requirement Pseudomonas sp.
2.1.2.7 pyridoxal 5'-phosphate Km: 0.01 mM at pH 7.5 Pseudomonas sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.2.7 0.002
-
 D-cycloserine
-
 Pseudomonas sp.
2.1.2.7 0.005
-
 L-Cycloserine
-
 Pseudomonas sp.
2.1.2.7 5.6
-
 L-serine
-
 Pseudomonas sp.