EC Number | General Stability | Organism |
---|---|---|
2.1.2.10 | lability of enzyme under the conditions of its purifications and storage | Gallus gallus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.2.10 | 0.0037 | - |
S-aminomethyldihydrolipoylprotein | - |
Gallus gallus | |
2.1.2.10 | 0.17 | - |
tetrahydrofolate | - |
Gallus gallus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.1.2.10 | mitochondrion | - |
Gallus gallus | 5739 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.2.10 | 37000 | 38000 | sedimentation equilibrium centrifugation, gel filtration | Gallus gallus |
2.1.2.10 | 41000 | - |
1 * 41000, SDS-PAGE | Gallus gallus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.2.10 | S-aminomethyldihydrolipoylprotein + (6S)-tetrahydrofolate | Gallus gallus | part of the glycine cleavage system | dihydrolipoylprotein + (6R)-5,10-methylenetetrahydrofolate + NH3 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.2.10 | Gallus gallus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.2.10 | 641fold purification | Gallus gallus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.1.2.10 | liver | - |
Gallus gallus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.1.2.10 | additional information | - |
- |
Gallus gallus |
EC Number | Storage Stability | Organism |
---|---|---|
2.1.2.10 | -20°C, 20 mM Tris-HCl buffer, pH 8.0, 10 mM 2-mercaptoethanol, 50% v/v glycerol, at least 2 months, stable | Gallus gallus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.2.10 | additional information | component of the glycine cleavage system, T-protein is associated with H-protein forming a complex which is composed of one molecule of each of them | Gallus gallus | ? | - |
? | |
2.1.2.10 | S-aminomethyldihydrolipoylprotein + (6S)-tetrahydrofolate | decarboxylated glycine moiety attached to H-protein + tetrahydrofolate as substrates | Gallus gallus | dihydrolipoylprotein + (6R)-5,10-methylenetetrahydrofolate + NH3 | - |
r | |
2.1.2.10 | S-aminomethyldihydrolipoylprotein + (6S)-tetrahydrofolate | strictly dependent on tetrahydrofolate | Gallus gallus | dihydrolipoylprotein + (6R)-5,10-methylenetetrahydrofolate + NH3 | - |
r | |
2.1.2.10 | S-aminomethyldihydrolipoylprotein + (6S)-tetrahydrofolate | part of the glycine cleavage system | Gallus gallus | dihydrolipoylprotein + (6R)-5,10-methylenetetrahydrofolate + NH3 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.2.10 | monomer | 1 * 41000, SDS-PAGE | Gallus gallus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.2.10 | 37 | - |
assay at | Gallus gallus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.2.10 | additional information | - |
pI: 9.8 | Gallus gallus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.2.10 | tetrahydrofolate | strictly dependent on tetrahydrofolate | Gallus gallus |