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Literature summary extracted from

  • Jones, G.H.
    Actinomycin synthesis in Streptomyces antibioticus: Enzymatic conversion of 3-hydroxyanthranilic acid to 4-methyl-3-hydroxyanthranilic acid (1987), J. Bacteriol., 169, 5575-5578.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.97 gene for the 88000 Mr PHS subunit cloned Streptomyces antibioticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.97 HEPES buffer
-
 Streptomyces antibioticus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.97 membrane intracellular membrane associated Streptomyces antibioticus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.97 36000
-
 1 * 36000, SDS-PAGE Streptomyces antibioticus
2.1.1.97 37000
-
 gel filtration, chromatography in 0.5 M NaCl Streptomyces antibioticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.97 S-adenosyl-L-methionine + 3-hydroxyanthranilate Streptomyces sp.
-
 S-adenosyl-L-homocysteine + 3-hydroxy-4-methylanthranilate
-
 ?
2.1.1.97 S-adenosyl-L-methionine + 3-hydroxyanthranilate Streptomyces anulatus
-
 S-adenosyl-L-homocysteine + 3-hydroxy-4-methylanthranilate
-
 ?
2.1.1.97 S-adenosyl-L-methionine + 3-hydroxyanthranilate Streptomyces antibioticus involved in the biosynthesis of the antibiotic actinomycin S-adenosyl-L-homocysteine + 3-hydroxy-4-methylanthranilate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.97 Streptomyces antibioticus
-
-
-
2.1.1.97 Streptomyces anulatus
-
-
-
2.1.1.97 Streptomyces sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.97
-
 Streptomyces antibioticus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.97 mycelium
-
 Streptomyces antibioticus
-

Storage Stability

EC Number Storage Stability Organism
2.1.1.97 -70°C, 5% glycerol, 50 mM sodium phosphate, pH 7.0, 10 mM Na2EDTA, 5 mM 2-mercaptoethanol, 1 mM phenylmethylsulfonyl fluoride, quite stable, can be frozen and thawed several times without appreciable loss of activity Streptomyces antibioticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.97 additional information does not catalyze the methylation of 3-hydroxy-DL-kynurenine Streptomyces antibioticus ?
-
 ?
2.1.1.97 S-adenosyl-L-methionine + 3-hydroxyanthranilate
-
 Streptomyces sp. S-adenosyl-L-homocysteine + 3-hydroxy-4-methylanthranilate
-
 ?
2.1.1.97 S-adenosyl-L-methionine + 3-hydroxyanthranilate
-
 Streptomyces anulatus S-adenosyl-L-homocysteine + 3-hydroxy-4-methylanthranilate
-
 ?
2.1.1.97 S-adenosyl-L-methionine + 3-hydroxyanthranilate involved in the biosynthesis of the antibiotic actinomycin Streptomyces antibioticus S-adenosyl-L-homocysteine + 3-hydroxy-4-methylanthranilate
-
 ?
2.1.1.97 S-adenosyl-L-methionine + 3-hydroxyanthranilic acid
-
 Streptomyces sp. S-adenosyl-L-homocysteine + 4-methyl-3-hydroxyanthranilic acid
-
 ?
2.1.1.97 S-adenosyl-L-methionine + 3-hydroxyanthranilic acid
-
 Streptomyces antibioticus S-adenosyl-L-homocysteine + 4-methyl-3-hydroxyanthranilic acid
-
 ?
2.1.1.97 S-adenosyl-L-methionine + 3-hydroxyanthranilic acid
-
 Streptomyces anulatus S-adenosyl-L-homocysteine + 4-methyl-3-hydroxyanthranilic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.97 monomer 1 * 36000, SDS-PAGE Streptomyces antibioticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.97 6 7 sodium phosphate buffer Streptomyces antibioticus
2.1.1.97 7 8 Tris buffer Streptomyces antibioticus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.97 6 9
-
 Streptomyces antibioticus