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Literature summary extracted from

  • Delk, A.S.; Nagle, D.P.; Rabinowitz, J.C.
    The methylenetetrahydrofolate-mediated biosynthesis of ribothymidine in the transfer-RNA of Streptococcus faecalis: incorporation of hydrogen from solvent into the methyl moiety (1979), Biochem. Biophys. Res. Commun., 86, 244-251.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.1.1.74 enzyme requires FAD+ for stability Enterococcus faecalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.74 5,10-methylenetetrahydrofolate + tRNA UpsiC + FADH2 Bacillus subtilis
-
 tetrahydrofolate + tRNA TpsiC + FAD
-
 ?
2.1.1.74 5,10-methylenetetrahydrofolate + tRNA UpsiC + FADH2 Enterococcus faecalis
-
 tetrahydrofolate + tRNA TpsiC + FAD
-
 ?
2.1.1.74 5,10-methylenetetrahydrofolate + tRNA UpsiC + FADH2 Bacillus cereus
-
 tetrahydrofolate + tRNA TpsiC + FAD
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.74 Bacillus cereus
-
-
-
2.1.1.74 Bacillus subtilis
-
-
-
2.1.1.74 Enterococcus faecalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.74
-
 Enterococcus faecalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.74 0.1
-
-
 Enterococcus faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.74 5,10-methylenetetrahydrofolate + tRNA UpsiC + FADH2
-
 Bacillus subtilis tetrahydrofolate + tRNA TpsiC + FAD
-
 ?
2.1.1.74 5,10-methylenetetrahydrofolate + tRNA UpsiC + FADH2
-
 Enterococcus faecalis tetrahydrofolate + tRNA TpsiC + FAD
-
 ?
2.1.1.74 5,10-methylenetetrahydrofolate + tRNA UpsiC + FADH2
-
 Bacillus cereus tetrahydrofolate + tRNA TpsiC + FAD
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.74 FADH2
-
 Bacillus subtilis
2.1.1.74 FADH2
-
 Enterococcus faecalis