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Literature summary extracted from

  • Delk, A.S.; Romeo, J.M.; Nagle, D.P.; Rabinowitz, J.C.
    Biosynthesis of ribothymidine in the transfer RNA of Streptococcus faecalis and Bacillus subtilis. A methylation of RNA involving 5,10-methylenetetrahydrofolate (1976), J. Biol. Chem., 251, 7649-7656.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.74 cytoplasm
-
 Enterococcus faecalis 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.74 5,10-methylenetetrahydrofolate + tRNA UpsiC + FADH2 Bacillus subtilis post-transcriptional biosynthesis of ribothymidine in the tRNA tetrahydrofolate + tRNA TpsiC + FAD
-
 ?
2.1.1.74 5,10-methylenetetrahydrofolate + tRNA UpsiC + FADH2 Enterococcus faecalis post-transcriptional biosynthesis of ribothymidine in the tRNA tetrahydrofolate + tRNA TpsiC + FAD
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.74 Bacillus subtilis
-
-
-
2.1.1.74 Enterococcus faecalis
-
-
-
2.1.1.74 no activity in Micrococcus luteus
-
 strains ATCC 4698 and ATCC12698, formerly Micrococcus lysodeikticus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.74 5,10-methylenetetrahydrofolate + tRNA UpsiC + FADH2
-
 Bacillus subtilis tetrahydrofolate + tRNA TpsiC + FAD
-
 ?
2.1.1.74 5,10-methylenetetrahydrofolate + tRNA UpsiC + FADH2
-
 Enterococcus faecalis tetrahydrofolate + tRNA TpsiC + FAD
-
 ?
2.1.1.74 5,10-methylenetetrahydrofolate + tRNA UpsiC + FADH2 post-transcriptional biosynthesis of ribothymidine in the tRNA Bacillus subtilis tetrahydrofolate + tRNA TpsiC + FAD
-
 ?
2.1.1.74 5,10-methylenetetrahydrofolate + tRNA UpsiC + FADH2 post-transcriptional biosynthesis of ribothymidine in the tRNA Enterococcus faecalis tetrahydrofolate + tRNA TpsiC + FAD
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.74 9
-
-
 Bacillus subtilis
2.1.1.74 9
-
-
 Enterococcus faecalis

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.74 NADPH
-
 Bacillus subtilis
2.1.1.74 NADPH
-
 Enterococcus faecalis