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Literature summary extracted from

  • Kawate, H.; Landis, D.M.; Loeb, L.A.
    Distribution of mutations in human thymidylate synthase yielding resistance to 5-fluorodeoxyuridine (2002), J. Biol. Chem., 277, 36304-36311.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.45 medicine enzyme is believed to be the major site of the cytotoxic action of the cancer chemotherapeutic drug 5-fluorouracil, development of inhibitors as clinical useful drugs Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.45 Escherichia coli NM522 cells used for cloning and library construction, Escherichia coli chi2913recA lacking TS used for expression by complementation Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.45
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.45 0.0007
-
 2'-deoxyuridylate mutant 362(T51S,K82Q,N171S) Homo sapiens
2.1.1.45 0.001
-
 2'-deoxyuridylate mutant Y258F Homo sapiens
2.1.1.45 0.0011
-
 2'-deoxyuridylate mutant 302(T53S,Y258F) Homo sapiens
2.1.1.45 0.002
-
 2'-deoxyuridylate wild type Homo sapiens
2.1.1.45 0.0026
-
 2'-deoxyuridylate mutant T51S Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.45 72000
-
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Homo sapiens biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism dihydrofolate + dTMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Homo sapiens P04818 human
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.45 recombinant enzyme Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.45 bone marrow
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 (6R)-N5,N10-methylene-5,6,7,8-tetrahydrofolate + 2'-deoxyuridylate
-
 Homo sapiens 7,8-dihydrofolate + 5-methyl-2'-deoxyuridylate
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Homo sapiens dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism Homo sapiens dihydrofolate + dTMP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.45 dimer homodimer Homo sapiens