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Literature summary extracted from

  • Golos, B.; Dzik, J.M.; Kazimierczuk, Z.; Ciesla, J.; Zielinski, Z.; Jankowska, J.; Kraszewski, A.; Stawinski, J.; Rode, W.; Shugar, D.
    Interaction of thymidylate synthase with the 5'-thiophosphates, 5'-dithiophosphates, 5'-H-phosphonates and 5'-S-thiosulfates of 2'-deoxyuridine, thymidine and 5-fluoro-2'-deoxyuridine (2001), Biol. Chem., 382, 1439-1445.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.45 medicine
-
 Mus musculus
2.1.1.45 medicine
-
 Pneumocystis carinii
2.1.1.45 medicine enzyme is believed to be the major site of the cytotoxic action of the cancer chemotherapeutic drug 5-fluorouracil, development of inhibitors as clinical useful drugs Homo sapiens
2.1.1.45 medicine enzyme is believed to be the major site of the cytotoxic action of the cancer chemotherapeutic drug 5-fluorouracil, development of inhibitors as clinical useful drugs Lacticaseibacillus casei

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.45
-
 Rattus norvegicus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.45
-
 Escherichia coli
2.1.1.45
-
 Rattus norvegicus
2.1.1.45
-
 Lacticaseibacillus casei

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Escherichia coli
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Homo sapiens
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Lacticaseibacillus casei
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Mus musculus
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Rattus norvegicus
2.1.1.45 dithiophosphate
-
 Mus musculus
2.1.1.45 FdUSSO3
-
 Mus musculus
2.1.1.45 fluorodeoxyuridine monophosphate
-
 Mus musculus
2.1.1.45 thiophosphate
-
 Mus musculus
2.1.1.45 TomudexR
-
 Homo sapiens
2.1.1.45 TomudexR
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.45 0.00315
-
 2'-deoxyuridylate
-
 Mus musculus
2.1.1.45 0.00365
-
 2'-deoxyuridylate
-
 Rattus norvegicus
2.1.1.45 0.00632
-
 2'-deoxyuridylate 5'-thiophosphate
-
 Mus musculus
2.1.1.45 0.0137
-
 2'-deoxyuridylate 5'-dithiophosphate
-
 Mus musculus
2.1.1.45 0.0155
-
 2'-deoxyuridylate 5'-dithiophosphate
-
 Rattus norvegicus
2.1.1.45 0.037
-
 2'-deoxyuridylate 5'-thiophosphate
-
 Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Mus musculus
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Rattus norvegicus
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Lacticaseibacillus casei
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Trichinella spiralis
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Trichinella pseudospiralis
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Escherichia coli biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Homo sapiens biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Pneumocystis carinii biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism dihydrofolate + dTMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Escherichia coli
-
-
-
2.1.1.45 Homo sapiens
-
 human
-
2.1.1.45 Lacticaseibacillus casei
-
-
-
2.1.1.45 Mus musculus
-
 L1210 cells
-
2.1.1.45 Pneumocystis carinii
-
-
-
2.1.1.45 Rattus norvegicus
-
-
-
2.1.1.45 Trichinella pseudospiralis
-
-
-
2.1.1.45 Trichinella spiralis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.45
-
 Mus musculus
2.1.1.45 recombinant enzyme, expressed in E. coli Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.45 liver
-
 Mus musculus
-
2.1.1.45 liver
-
 Homo sapiens
-
2.1.1.45 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Mus musculus dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Mus musculus dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Escherichia coli dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Homo sapiens dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Rattus norvegicus dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Rattus norvegicus dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Lacticaseibacillus casei dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Lacticaseibacillus casei dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Pneumocystis carinii dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Trichinella spiralis dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Trichinella spiralis dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Trichinella pseudospiralis dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Trichinella pseudospiralis dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism Escherichia coli dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism Homo sapiens dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism Pneumocystis carinii dihydrofolate + dTMP
-
 ?
2.1.1.45 N5,10-methylenetetrahydrofolate + 2'-deoxyuridine 5'-dithiophosphate
-
 Mus musculus 7,8-dihydrofolate + ?
-
 ?
2.1.1.45 N5,10-methylenetetrahydrofolate + 2'-deoxyuridine 5'-dithiophosphate
-
 Rattus norvegicus 7,8-dihydrofolate + ?
-
 ?
2.1.1.45 N5,10-methylenetetrahydrofolate + 2'-deoxyuridine 5'-thiophosphate
-
 Mus musculus 7,8-dihydrofolate + ?
-
 ?
2.1.1.45 N5,10-methylenetetrahydrofolate + 2'-deoxyuridine 5'-thiophosphate
-
 Rattus norvegicus 7,8-dihydrofolate + ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.45 6.5 7
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.45 4 8
-
 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.45 0.0017
-
 thiophosphate
-
 Mus musculus
2.1.1.45 0.0084
-
 dithiophosphate
-
 Mus musculus