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Literature summary extracted from

  • Liang, P.H.; Anderson, K.S.
    Substrate channeling and domain-domain interactions in bifunctional thymidylate synthase-dihydrofolate reductase (1998), Biochemistry, 37, 12195-12205.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.45 clone harbors the pO2CLSA-4 plasmid expressed in an Escherichia coli Rue 10 expression vector Leishmania major

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.45
-
 Leishmania major

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Escherichia coli
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Leishmania major

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Escherichia coli
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Leishmania major
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Toxoplasma gondii
-
 dihydrofolate + dTMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Escherichia coli
-
-
-
2.1.1.45 Leishmania major
-
 formerly called Leishmania tropica
-
2.1.1.45 Toxoplasma gondii
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 (6S)-tetrahydropteroylglutamate + 2-deoxyuridylate
-
 Leishmania major 7,8-dihydropteroylglutamate + thymidine phosphate
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Escherichia coli dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Escherichia coli dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Leishmania major dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Leishmania major dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Toxoplasma gondii dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Toxoplasma gondii dihydrofolate + dTMP
-
 r
2.1.1.45 additional information
-
 Escherichia coli ?
-
 ?
2.1.1.45 additional information bifunctional enzyme dihydrofolate reductase-thymidylate synthase Leishmania major ?
-
 ?
2.1.1.45 additional information bifunctional enzyme dihydrofolate reductase-thymidylate synthase Toxoplasma gondii ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.45 1.67
-
 2'-deoxyuridine
-
 Leishmania major