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Literature summary extracted from

  • Chiericatti, G.; Santi, D.V.
    Aspartate 221 of thymidylate synthase is involved in folate cofactor binding and in catalysis (1998), Biochemistry, 37, 9038-9042.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.45
-
 Lacticaseibacillus casei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.45 0.005
-
 2'-deoxyuridylate wild-type Lacticaseibacillus casei
2.1.1.45 0.0057
-
 5-bromo-2'-deoxyuridylate
-
 Lacticaseibacillus casei
2.1.1.45 0.0057
-
 5-bromo-2'-deoxyuridylate wild-type Escherichia coli
2.1.1.45 0.0063
-
 2'-deoxyuridylate
-
 Lacticaseibacillus casei
2.1.1.45 0.0063
-
 2'-deoxyuridylate mutant D221S Escherichia coli
2.1.1.45 0.011
-
 2'-deoxyuridylate
-
 Lacticaseibacillus casei
2.1.1.45 0.011
-
 2'-deoxyuridylate mutant D221E Escherichia coli
2.1.1.45 0.015
-
 2'-deoxyuridylate
-
 Lacticaseibacillus casei
2.1.1.45 0.015
-
 2'-deoxyuridylate mutant D221C Escherichia coli
2.1.1.45 0.02
-
 5-bromo-2'-deoxyuridylate
-
 Lacticaseibacillus casei
2.1.1.45 0.02
-
 5-bromo-2'-deoxyuridylate mutant D221A Escherichia coli
2.1.1.45 0.025
-
 5-bromo-2'-deoxyuridylate
-
 Lacticaseibacillus casei
2.1.1.45 0.025
-
 5-bromo-2'-deoxyuridylate mutant D221C Escherichia coli
2.1.1.45 0.027
-
 5-bromo-2'-deoxyuridylate
-
 Lacticaseibacillus casei
2.1.1.45 0.027
-
 5-bromo-2'-deoxyuridylate mutant D221S Escherichia coli
2.1.1.45 0.045
-
 5-bromo-2'-deoxyuridylate
-
 Lacticaseibacillus casei
2.1.1.45 0.045
-
 5-bromo-2'-deoxyuridylate mutant D221N Escherichia coli
2.1.1.45 0.046
-
 5-bromo-2'-deoxyuridylate
-
 Lacticaseibacillus casei
2.1.1.45 0.046
-
 5-bromo-2'-deoxyuridylate mutant D221E Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Escherichia coli
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Lacticaseibacillus casei
-
 dihydrofolate + dTMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Escherichia coli
-
-
-
2.1.1.45 Lacticaseibacillus casei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.45 mutant enzymes D221A, N, R, F, C, E, and S Lacticaseibacillus casei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + 5'-fluoro-2'-deoxyuridine 5'-phosphate some mutants are capable of forming covalent thymidylate synthase-5-fluoro-dUMP-methylenetetrahydrofolate complex Lacticaseibacillus casei 7,8-dihydrofolate + ?
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Escherichia coli dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Escherichia coli dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Lacticaseibacillus casei dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Lacticaseibacillus casei dihydrofolate + dTMP
-
 r
2.1.1.45 5-bromo-2'-deoxyuridine 5'-monophosphate + ? dehalogenation Escherichia coli ?
-
 ?
2.1.1.45 5-bromo-2'-deoxyuridine 5'-monophosphate + ? dehalogenation Lacticaseibacillus casei ?
-
 ?