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Literature summary extracted from

  • Spencer, H.T.; Villafranca, J.E.; Appleman, J.R.
    Kinetic scheme for thymidylate synthase from Escherichia coli: determination from measurements of ligand binding, primary and secondary isotope effects, and pre-steady-state catalysis (1997), Biochemistry, 36, 4212-4222.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.45 medicine
-
 Mus musculus
2.1.1.45 medicine enzyme is believed to be the major site of the cytotoxic action of the cancer chemotherapeutic drug 5-fluorouracil, development of inhibitors as clinical useful drugs Homo sapiens
2.1.1.45 medicine enzyme is believed to be the major site of the cytotoxic action of the cancer chemotherapeutic drug 5-fluorouracil, development of inhibitors as clinical useful drugs Lacticaseibacillus casei
2.1.1.45 medicine enzyme is believed to be the major site of the cytotoxic action of the cancer chemotherapeutic drug 5-fluorouracil, development of inhibitors as clinical useful drugs Tequatrovirus T4

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.45
-
 Mus musculus
2.1.1.45
-
 Escherichia coli
2.1.1.45
-
 Homo sapiens
2.1.1.45
-
 Lacticaseibacillus casei
2.1.1.45
-
 Tequatrovirus T4
2.1.1.45
-
 Candida albicans

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.45 10-propargyl-5,8-dideazafolate
-
 Escherichia coli
2.1.1.45 5-Mercapto-2'-deoxyuridylic acid
-
 Homo sapiens
2.1.1.45 dihydrofolate
-
 Escherichia coli
2.1.1.45 thymidine phosphate
-
 Escherichia coli
2.1.1.45 thymidine phosphate
-
 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.45 0.011
-
 methylenetetrahydrofolate
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.45 60000
-
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Mus musculus
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Lacticaseibacillus casei
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Tequatrovirus T4
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Candida albicans
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Escherichia coli biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Homo sapiens biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism dihydrofolate + dTMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Candida albicans
-
-
-
2.1.1.45 Escherichia coli
-
-
-
2.1.1.45 Homo sapiens
-
-
-
2.1.1.45 Lacticaseibacillus casei
-
-
-
2.1.1.45 Mus musculus
-
-
-
2.1.1.45 Tequatrovirus T4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.45
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP
-
 Escherichia coli 7,8-dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Mus musculus dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Mus musculus dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Escherichia coli dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Homo sapiens dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Lacticaseibacillus casei dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Lacticaseibacillus casei dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Tequatrovirus T4 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Tequatrovirus T4 dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Candida albicans dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Candida albicans dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism Escherichia coli dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism Homo sapiens dihydrofolate + dTMP
-
 ?
2.1.1.45 5-bromo-2'-deoxyuridine 5'-monophosphate + ? dehalogenation Mus musculus ?
-
 ?
2.1.1.45 5-bromo-2'-deoxyuridine 5'-monophosphate + ? dehalogenation Escherichia coli ?
-
 ?
2.1.1.45 5-bromo-2'-deoxyuridine 5'-monophosphate + ? dehalogenation Lacticaseibacillus casei ?
-
 ?
2.1.1.45 5-iodo-2'-deoxyuridine 5'-monophosphate + ?
-
 Mus musculus ?
-
 ?
2.1.1.45 5-iodo-2'-deoxyuridine 5'-monophosphate + ?
-
 Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.45 dimer
-
 Mus musculus
2.1.1.45 dimer
-
 Escherichia coli
2.1.1.45 dimer
-
 Homo sapiens
2.1.1.45 dimer
-
 Lacticaseibacillus casei
2.1.1.45 dimer
-
 Tequatrovirus T4

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.45 0.00001
-
 5-Mercapto-2'-deoxyuridylic acid
-
 Homo sapiens