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Literature summary extracted from

  • Carlson, J.H.; Hill, S.A.
    Identification and characterization of thymidylate synthase from Neisseria gonorrhoeae (1997), FEMS Microbiol. Lett., 151, 225-230.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.45 thyA gene cloned, sequenced and expressed in Escherichia coli KLC1597, transformation with pBr322 Neisseria gonorrhoeae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Haemophilus influenzae
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Bacillus subtilis
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Neisseria gonorrhoeae biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Neisseria gonorrhoeae MS11 biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism dihydrofolate + dTMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Bacillus subtilis
-
-
-
2.1.1.45 Haemophilus influenzae
-
-
-
2.1.1.45 Neisseria gonorrhoeae
-
-
-
2.1.1.45 Neisseria gonorrhoeae MS11
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.45
-
 Neisseria gonorrhoeae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Haemophilus influenzae dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Haemophilus influenzae dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Bacillus subtilis dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Bacillus subtilis dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Neisseria gonorrhoeae dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism Neisseria gonorrhoeae dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Neisseria gonorrhoeae MS11 dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism Neisseria gonorrhoeae MS11 dihydrofolate + dTMP
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.45 0.000001
-
 5-fluoro-2'-deoxyuridylic acid
-
 Bacillus subtilis