EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.45 | cloned and overexpressed in Escherichia coli | Lacticaseibacillus casei |
2.1.1.45 | plasmid pSCTS9, synthetic TS gene in pUC18 | Lacticaseibacillus casei |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.1.1.45 | - |
Lacticaseibacillus casei |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.1.1.45 | T155C/E188C/C244T | formation of 2 disulfide crosslinks across the subunit interface results in dramatic stabilization of the overall structure of the protein, retention of secondary structure at temperatures as high as 90°C, engineering of an intersubunit crosslink yields a fully active enzyme | Lacticaseibacillus casei |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.45 | 5-fluoro-2'-deoxyuridylic acid | - |
Lacticaseibacillus casei |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.45 | 35000 | - |
2 * 35000, SDS-PAGE | Lacticaseibacillus casei |
2.1.1.45 | 36620 | - |
calculated molecular mass based on the sequence | Lacticaseibacillus casei |
2.1.1.45 | 71000 | - |
analytical ultracentrifugation | Lacticaseibacillus casei |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | Lacticaseibacillus casei | - |
dihydrofolate + dTMP | - |
? | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | Tequatrovirus T4 | - |
dihydrofolate + dTMP | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.45 | Lacticaseibacillus casei | - |
- |
- |
2.1.1.45 | Tequatrovirus T4 | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
? | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
r | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | - |
Tequatrovirus T4 | dihydrofolate + dTMP | - |
? | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | - |
Tequatrovirus T4 | dihydrofolate + dTMP | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.1.45 | dimer | - |
Tequatrovirus T4 |
2.1.1.45 | dimer | 2 * 35000, SDS-PAGE | Lacticaseibacillus casei |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.1.45 | 55 | - |
mutant enzyme, 2 disulfide bridges across the dimer interface of the enzyme engineered | Lacticaseibacillus casei |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.1.45 | 30 | 70 | mutant enzyme, 2 disulfide bridges across the dimer interface of the enzyme engineered | Lacticaseibacillus casei |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.1.45 | 52 | - |
wild-type enzyme, precipitates at 52°C | Lacticaseibacillus casei |
2.1.1.45 | 90 | - |
mutant enzyme remains soluble, enhancement of activity is observed up to 60°C, possesses appreciable enzymatic activity at 65°C and retains secondary structure even at 90°C | Lacticaseibacillus casei |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.45 | 0.000005 | - |
5-fluoro-2'-deoxyuridylic acid | - |
Lacticaseibacillus casei |