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Literature summary extracted from

  • So, N.N.C.; Wong, P.C.L.; Ko, R.C.
    Angiostrongylus cantonensis: Characterization of thymidylate synthetase (1994), Exp. Parasitol., 79, 526-535.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.45 medicine
-
 Mus musculus
2.1.1.45 medicine
-
 Homo sapiens
2.1.1.45 medicine
-
 Crithidia fasciculata
2.1.1.45 medicine
-
 Plasmodium falciparum
2.1.1.45 medicine development of effective chemotherapy of the eosinophilic meningoencephalitis angiostrongylosis Angiostrongylus cantonensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.45
-
 Candida albicans

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Angiostrongylus cantonensis
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Homo sapiens
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Leishmania major
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Mus musculus
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Plasmodium falciparum
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Rattus norvegicus
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Saccharomyces cerevisiae
2.1.1.45 aminofolate
-
 Angiostrongylus cantonensis
2.1.1.45 dihydrofolate
-
 Angiostrongylus cantonensis
2.1.1.45 methotrexate
-
 Angiostrongylus cantonensis
2.1.1.45 methotrexate
-
 Homo sapiens
2.1.1.45 methotrexate
-
 Saccharomyces cerevisiae
2.1.1.45 thymidine phosphate
-
 Angiostrongylus cantonensis
2.1.1.45 thymidine phosphate
-
 Bos taurus
2.1.1.45 thymidine phosphate
-
 Mus musculus
2.1.1.45 trimethoprim
-
 Angiostrongylus cantonensis
2.1.1.45 trimethoprim
-
 Crithidia fasciculata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.45 0.01
-
 2'-deoxyuridylate
-
 Angiostrongylus cantonensis
2.1.1.45 0.185
-
 5,10-methylenetetrahydrofolate
-
 Angiostrongylus cantonensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.45 31000
-
 2 * 31000, SDS-PAGE Angiostrongylus cantonensis
2.1.1.45 66000
-
 gel filtration Homo sapiens
2.1.1.45 66000
-
 gel filtration Angiostrongylus cantonensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Gallus gallus
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Rattus norvegicus
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Sus scrofa
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Saccharomyces cerevisiae
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Bos taurus
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Crithidia fasciculata
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Aedes aegypti
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Candida albicans
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Plasmodium falciparum
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Leishmania major
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Brugia pahangi
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Toxoplasma gondii
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Mus musculus key function in DNA synthesis, repair and cell division dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Homo sapiens key function in DNA synthesis, repair and cell division dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Angiostrongylus cantonensis key function in DNA synthesis, repair and cell division dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Homo sapiens biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Angiostrongylus cantonensis biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism dihydrofolate + dTMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Aedes aegypti
-
-
-
2.1.1.45 Angiostrongylus cantonensis
-
 metastrongyloid nematode, synonym Parastrongylus
-
2.1.1.45 Bos taurus
-
 calf
-
2.1.1.45 Brugia pahangi
-
-
-
2.1.1.45 Candida albicans
-
-
-
2.1.1.45 Crithidia fasciculata
-
-
-
2.1.1.45 Gallus gallus
-
 chicken
-
2.1.1.45 Homo sapiens
-
 human
-
2.1.1.45 Leishmania major
-
-
-
2.1.1.45 Mus musculus
-
 Ehrlich ascites carcinoma cells
-
2.1.1.45 Plasmodium falciparum
-
-
-
2.1.1.45 Rattus norvegicus
-
-
-
2.1.1.45 Saccharomyces cerevisiae
-
-
-
2.1.1.45 Sus scrofa
-
 pig
-
2.1.1.45 Toxoplasma gondii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.45
-
 Gallus gallus
2.1.1.45
-
 Homo sapiens
2.1.1.45
-
 Bos taurus
2.1.1.45
-
 Candida albicans
2.1.1.45
-
 Plasmodium falciparum
2.1.1.45
-
 Angiostrongylus cantonensis
2.1.1.45 recombinant enzyme Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.45 blast cell
-
 Homo sapiens
-
2.1.1.45 fetus
-
 Rattus norvegicus
-
2.1.1.45 fetus
-
 Sus scrofa
-
2.1.1.45 fibroblast
-
 Mus musculus
-
2.1.1.45 heart parasitic helminth, obtained from the pulmonary hearts and vessels of infected Wistar albino rats Angiostrongylus cantonensis
-
2.1.1.45 liver
-
 Mus musculus
-
2.1.1.45 liver
-
 Homo sapiens
-
2.1.1.45 liver
-
 Rattus norvegicus
-
2.1.1.45 liver
-
 Sus scrofa
-
2.1.1.45 thymus
-
 Mus musculus
-
2.1.1.45 thymus
-
 Homo sapiens
-
2.1.1.45 thymus
-
 Sus scrofa
-
2.1.1.45 thymus
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.45 0.0003367
-
-
 Angiostrongylus cantonensis

Storage Stability

EC Number Storage Stability Organism
2.1.1.45 -70°C preparation with 0.1% bovine serum albumin is stable up to 8 weeks of storage Angiostrongylus cantonensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Gallus gallus dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Gallus gallus dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Mus musculus dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Homo sapiens dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Rattus norvegicus dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Rattus norvegicus dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Sus scrofa dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Sus scrofa dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Saccharomyces cerevisiae dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Saccharomyces cerevisiae dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Bos taurus dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Bos taurus dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Crithidia fasciculata dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Crithidia fasciculata dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Aedes aegypti dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Aedes aegypti dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Candida albicans dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Candida albicans dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Plasmodium falciparum dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Plasmodium falciparum dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Leishmania major dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Leishmania major dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Brugia pahangi dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Brugia pahangi dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Angiostrongylus cantonensis dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Toxoplasma gondii dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Toxoplasma gondii dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP key function in DNA synthesis, repair and cell division Mus musculus dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP key function in DNA synthesis, repair and cell division Homo sapiens dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP key function in DNA synthesis, repair and cell division Angiostrongylus cantonensis dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism Homo sapiens dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism Angiostrongylus cantonensis dihydrofolate + dTMP
-
 ?
2.1.1.45 additional information
-
 Homo sapiens ?
-
 ?
2.1.1.45 additional information bifunctional enzyme dihydrofolate reductase-thymidylate synthase Plasmodium falciparum ?
-
 ?
2.1.1.45 additional information bifunctional enzyme dihydrofolate reductase-thymidylate synthase Leishmania major ?
-
 ?
2.1.1.45 additional information bifunctional enzyme dihydrofolate reductase-thymidylate synthase Toxoplasma gondii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.45 dimer
-
 Mus musculus
2.1.1.45 dimer
-
 Homo sapiens
2.1.1.45 dimer
-
 Rattus norvegicus
2.1.1.45 dimer
-
 Saccharomyces cerevisiae
2.1.1.45 dimer
-
 Bos taurus
2.1.1.45 dimer
-
 Plasmodium falciparum
2.1.1.45 dimer
-
 Leishmania major
2.1.1.45 dimer 2 * 31000, SDS-PAGE Angiostrongylus cantonensis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.45 0.000021
-
 5-fluoro-2'-deoxyuridylic acid 50 nM Angiostrongylus cantonensis
2.1.1.45 0.0000235
-
 5-fluoro-2'-deoxyuridylic acid
-
 Angiostrongylus cantonensis
2.1.1.45 0.000026
-
 5-fluoro-2'-deoxyuridylic acid 200 nM Angiostrongylus cantonensis
2.1.1.45 0.000833
-
 trimethoprim 1.0 mM Angiostrongylus cantonensis
2.1.1.45 0.00087
-
 trimethoprim 2.0 mM Angiostrongylus cantonensis
2.1.1.45 0.001
-
 thymidine phosphate 0.25 mM, noncompetitive inhibition, 5,10-mehylenetetrahydrofolate as variable substrate Angiostrongylus cantonensis
2.1.1.45 0.00124
-
 thymidine phosphate 1.0 mM, noncompetitive inhibition, 5,10-methylenetetrahydrofolate as variable substrate Angiostrongylus cantonensis
2.1.1.45 0.0014
-
 dihydrofolate 1.0 mM, noncompetitive inhibition, 5,10-methylenetetrahydrofolate as variable substrate Angiostrongylus cantonensis
2.1.1.45 0.00178
-
 dihydrofolate 5.0 mM, noncompetitive inhibition, 5,10-methylenetetrahydrofolate as variable substrate Angiostrongylus cantonensis
2.1.1.45 0.00294
-
 dihydrofolate 0.5 mM, noncompetitive inhibition, dUMP as variable substrate Angiostrongylus cantonensis
2.1.1.45 0.00303
-
 dihydrofolate 1.0 mM, noncompetitive inhibition, dUMP as variable substrate Angiostrongylus cantonensis
2.1.1.45 0.238
-
 methotrexate 0.05 mM Angiostrongylus cantonensis
2.1.1.45 0.267
-
 methotrexate 0.02 mM Angiostrongylus cantonensis
2.1.1.45 0.3125
-
 thymidine phosphate 0.25 mM, competitive inhibition, dUMP as variable substrate Angiostrongylus cantonensis
2.1.1.45 0.333
-
 thymidine phosphate
-
 Angiostrongylus cantonensis
2.1.1.45 0.406
-
 methotrexate 0.5 mM Angiostrongylus cantonensis
2.1.1.45 0.413
-
 methotrexate 1.0 mM Angiostrongylus cantonensis
2.1.1.45 0.852
-
 trimethoprim
-
 Angiostrongylus cantonensis
2.1.1.45 5
-
 aminofolate 1.0 mM Angiostrongylus cantonensis
2.1.1.45 5.2
-
 aminofolate 5.0 mM Angiostrongylus cantonensis