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Literature summary extracted from

  • Balinska, M.; Rhee, M.; Whiteley, J.M.; Priest, D.G.; Galivan, J.
    Inhibition of mammalian thymidylate synthase by 10-formyltetrahydropteroylpolyglutamate (1991), Arch. Biochem. Biophys., 284, 219-222.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.45 10-formyl-7,8 dihydrofolate-tetra-gamma-L-glutamate
-
 Homo sapiens
2.1.1.45 10-formyldihydropteroylglutamate
-
 Rattus norvegicus
2.1.1.45 10-formyldihydropteroylheptaglutamate
-
 Rattus norvegicus
2.1.1.45 10-formyldihydropteroylpentaglutamate
-
 Homo sapiens
2.1.1.45 10-formyltetrahydrofolate
-
 Rattus norvegicus
2.1.1.45 10-Formyltetrahydrofolylheptaglutamate
-
 Rattus norvegicus
2.1.1.45 10-Formyltetrahydrofolylmonoglutamate
-
 Rattus norvegicus
2.1.1.45 10-formyltetrahydropteroylheptaglutamate
-
 Rattus norvegicus
2.1.1.45 10-formyltetrahydropteroylmonoglutamate
-
 Mus musculus
2.1.1.45 10-formyltetrahydropteroylpolyglutamate
-
 Rattus norvegicus
2.1.1.45 5,10-methenyltetrahydrofolylhexa-gamma-L-glutamate
-
 Homo sapiens
2.1.1.45 dihydropteroylpentaglutamate
-
 Homo sapiens
2.1.1.45 N10-formyltetrahydrofolate
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.45 0.00068
-
 5,10-formyltetrahydropteroylheptaglutamate
-
 Rattus norvegicus
2.1.1.45 0.00074
-
 5,10-methenyltetrahydropteroylpentaglutamate
-
 Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Mus musculus
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Homo sapiens
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Rattus norvegicus
-
 dihydrofolate + dTMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Homo sapiens
-
 HeLa cells
-
2.1.1.45 Homo sapiens
-
 MCF-7 breast cancer cells
-
2.1.1.45 Mus musculus
-
 Ehrlich ascites carcinoma cells
-
2.1.1.45 Rattus norvegicus
-
 rat
-
2.1.1.45 Rattus norvegicus
-
 H35 hepatoma cells
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.45
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.45 liver
-
 Mus musculus
-
2.1.1.45 liver
-
 Homo sapiens
-
2.1.1.45 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 5,10-formyltetrahydropteroylheptaglutamate + dUMP
-
 Rattus norvegicus ?
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Mus musculus dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Mus musculus dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Homo sapiens dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Homo sapiens dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Rattus norvegicus dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Rattus norvegicus dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolylheptaglutamate + 2'-deoxyuridylate
-
 Rattus norvegicus 7,8-dihydrofolylheptaglutamate + thymidine phosphate
-
 r
2.1.1.45 5,10-methylenetetrahydrofolylpentaglutamate + 2'-deoxyuridylate
-
 Rattus norvegicus 7,8-dihydrofolylpentaglutamate + thymidine phosphate
-
 r
2.1.1.45 5,10-methylenetetrahydropteroylheptaglutamate + 2'-deoxyuridylate
-
 Homo sapiens 7,8-dihydropteroylheptaglutamate + thymidine phosphate
-
 r
2.1.1.45 5,10-methylenetetrahydropteroylheptaglutamate + 2'-deoxyuridylate
-
 Rattus norvegicus 7,8-dihydropteroylheptaglutamate + thymidine phosphate
-
 r
2.1.1.45 5,10-methylenetetrahydropteroylpentaglutamate + 2'-deoxyuridylate
-
 Homo sapiens 7,8-dihydropteroylpentaglutamate + thymidine phosphate
-
 r
2.1.1.45 5,10-methylenetetrahydropteroylpentaglutamate + 2'-deoxyuridylate
-
 Rattus norvegicus 7,8-dihydropteroylpentaglutamate + thymidine phosphate
-
 r
2.1.1.45 [6R,S]-5,10-methylenetetrahydropteroylglutamate + 2'-deoxyuridylate
-
 Mus musculus ?
-
 r

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.45 0.0001
-
 10-Formyltetrahydrofolylheptaglutamate
-
 Rattus norvegicus
2.1.1.45 0.0007
-
 10-formyldihydropteroylheptaglutamate
-
 Rattus norvegicus
2.1.1.45 0.0016
-
 10-formyldihydropteroylpentaglutamate
-
 Homo sapiens
2.1.1.45 0.0024
-
 10-Formyltetrahydrofolylmonoglutamate
-
 Rattus norvegicus
2.1.1.45 0.0075
-
 10-formyldihydropteroylglutamate
-
 Rattus norvegicus
2.1.1.45 0.02
-
 10-formyltetrahydropteroylmonoglutamate
-
 Mus musculus