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Literature summary extracted from

  • Pattanakitsakul, S.N.; Ruenwongsa, P.
    Characterization of thymidylate synthetase and dihydrofolate reductase from Plasmodium berghei (1984), Int. J. Parasitol., 14, 513-520.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.45 medicine
-
 Homo sapiens
2.1.1.45 medicine
-
 Crithidia fasciculata
2.1.1.45 medicine target enzyme for antitumor and antibacterial agents Plasmodium berghei
2.1.1.45 medicine target enzyme for malarial chemotherapy Plasmodium berghei

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Plasmodium berghei
2.1.1.45 KCl
-
 Plasmodium berghei
2.1.1.45 methotrexate
-
 Homo sapiens
2.1.1.45 methotrexate
-
 Plasmodium berghei
2.1.1.45 NaCl
-
 Plasmodium berghei
2.1.1.45 Urea
-
 Plasmodium berghei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.45 0.0714
-
 2'-deoxyuridylate
-
 Plasmodium berghei
2.1.1.45 0.3125
-
 5,10-methylenetetrahydrofolate
-
 Plasmodium berghei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.45 65000
-
 2 * 65000, SDS-PAGE Plasmodium berghei
2.1.1.45 132000
-
 gel filtration Plasmodium berghei
2.1.1.45 150000
-
 gel filtration Crithidia fasciculata
2.1.1.45 150000
-
 gel filtration Plasmodium berghei
2.1.1.45 150000
-
 gel filtration Leishmania major

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Crithidia fasciculata
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Streptococcus pneumoniae
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Leishmania major
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Escherichia coli key function in DNA synthesis, repair and cell division dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Homo sapiens key function in DNA synthesis, repair and cell division dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Lacticaseibacillus casei key function in DNA synthesis, repair and cell division dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Plasmodium berghei key function in DNA synthesis, repair and cell division dihydrofolate + dTMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Crithidia fasciculata
-
-
-
2.1.1.45 Escherichia coli
-
-
-
2.1.1.45 Homo sapiens
-
 human
-
2.1.1.45 Lacticaseibacillus casei
-
-
-
2.1.1.45 Leishmania major
-
-
-
2.1.1.45 Plasmodium berghei
-
-
-
2.1.1.45 Streptococcus pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.45
-
 Homo sapiens
2.1.1.45 partially, copurified with dihydrofolate reductase Plasmodium berghei

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.45 blast cell from patients with acute myelocytic leukemia Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.45 0.00235
-
-
 Plasmodium berghei

Storage Stability

EC Number Storage Stability Organism
2.1.1.45 4°C, enzyme loses half of its activity upon storage for a few days Plasmodium berghei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Escherichia coli dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Homo sapiens dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Crithidia fasciculata dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Crithidia fasciculata dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Streptococcus pneumoniae dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Streptococcus pneumoniae dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Lacticaseibacillus casei dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Plasmodium berghei dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Leishmania major dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Leishmania major dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP key function in DNA synthesis, repair and cell division Escherichia coli dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP key function in DNA synthesis, repair and cell division Homo sapiens dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP key function in DNA synthesis, repair and cell division Lacticaseibacillus casei dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP key function in DNA synthesis, repair and cell division Plasmodium berghei dihydrofolate + dTMP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.45 dimer
-
 Escherichia coli
2.1.1.45 dimer
-
 Homo sapiens
2.1.1.45 dimer
-
 Streptococcus pneumoniae
2.1.1.45 dimer
-
 Lacticaseibacillus casei
2.1.1.45 dimer
-
 Leishmania major
2.1.1.45 dimer 2 * 65000, SDS-PAGE Plasmodium berghei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.45 6.5 8
-
 Plasmodium berghei

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.1.1.45 5 10 stable in this range but loses activity completely below pH 3.5 or above pH 11.5 Plasmodium berghei

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.45 0.00005
-
 5-fluoro-2'-deoxyuridylic acid
-
 Plasmodium berghei
2.1.1.45 0.103
-
 methotrexate uncompetitive with dUMP Plasmodium berghei