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Literature summary extracted from

  • Banerjee, C.K.; Bennett, L.L.; Brockman, R.W.; Sani, B.P.; Temple, C.
    A convenient procedure for purification of thymidylate synthase from L1210 cells (1982), Anal. Biochem., 121, 275-280.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.45 medicine
-
 Mus musculus
2.1.1.45 medicine enzyme is believed to be the major site of the cytotoxic action of the cancer chemotherapeutic drug 5-fluorouracil, development of inhibitors as clinical useful drugs Homo sapiens
2.1.1.45 medicine enzyme is believed to be the major site of the cytotoxic action of the cancer chemotherapeutic drug 5-fluorouracil, development of inhibitors as clinical useful drugs Lacticaseibacillus casei

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.45 10-formyl folic acid
-
 Mus musculus
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Homo sapiens
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Lacticaseibacillus casei
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.45 38000
-
 2 * 38000, SDS-PAGE Mus musculus
2.1.1.45 38500
-
 2 * 38500, SDS-PAGE Mus musculus
2.1.1.45 58000
-
-
 Gallus gallus
2.1.1.45 76000
-
-
 Homo sapiens
2.1.1.45 76000
-
 L1210 cells, purified enzyme, gel filtration Mus musculus
2.1.1.45 78000
-
 L1210 cells, gel filtration Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Gallus gallus
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Mus musculus
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Homo sapiens
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Lacticaseibacillus casei
-
 dihydrofolate + dTMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Gallus gallus
-
 chicken
-
2.1.1.45 Homo sapiens
-
-
-
2.1.1.45 Lacticaseibacillus casei
-
-
-
2.1.1.45 Mus musculus
-
 L1210 cells
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.45
-
 Gallus gallus
2.1.1.45
-
 Lacticaseibacillus casei
2.1.1.45 L1210 cells Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.45 blast cell from patients with acute myelocytic leukemia Homo sapiens
-
2.1.1.45 embryo
-
 Gallus gallus
-
2.1.1.45 peritoneum L1210 leukemia cells grown in the peritoneal cavity of CDF1 mice Mus musculus
-
2.1.1.45 thymus
-
 Mus musculus
-
2.1.1.45 thymus
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Gallus gallus dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Gallus gallus dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Mus musculus dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Mus musculus dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Homo sapiens dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Homo sapiens dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Lacticaseibacillus casei dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Lacticaseibacillus casei dihydrofolate + dTMP
-
 r

Subunits

EC Number Subunits Comment Organism
2.1.1.45 dimer 2 * 38500, SDS-PAGE Mus musculus
2.1.1.45 dimer 2 * 38000, SDS-PAGE Mus musculus