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Literature summary extracted from

  • Priest, D.G.; Doig, M.T.; Hynes, J.B.
    Purification of mouse liver thymidylate synthetase by affinity chromatography using 10-methyl-5,8-dideazafolate as the affinant (1981), Experientia, 37, 119-120.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.45 medicine
-
 Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.45 35000
-
 2 * 35000, SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Mus musculus
-
 dihydrofolate + dTMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Mus musculus
-
 L1210 cells
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.45
-
 Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.45 liver neonatal mouse liver Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.45 0.01
-
-
 Mus musculus

Storage Stability

EC Number Storage Stability Organism
2.1.1.45 -20°C, can be stored in 10% glycerol, 0.05 M Tris-HCl, pH7.4 and 10 mM 2-mercaptoethanol for 30 days with a loss of approximately 50% activity Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Mus musculus dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Mus musculus dihydrofolate + dTMP
-
 r

Subunits

EC Number Subunits Comment Organism
2.1.1.45 dimer 2 * 35000, SDS-PAGE Mus musculus