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Literature summary extracted from

  • Bisson, L.F.; Thorner, J.
    Thymidylate synthetase from Saccharomyces cerevisiae. Purification and enzymic properties (1981), J. Biol. Chem., 256, 12456-12462.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.45 5-bromo-2'-deoxyuridylic acid
-
 Saccharomyces cerevisiae
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Enterococcus faecalis
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Homo sapiens
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Lacticaseibacillus casei
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Mus musculus
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Saccharomyces cerevisiae
2.1.1.45 aminopterin
-
 Saccharomyces cerevisiae
2.1.1.45 Cibacron blue
-
 Saccharomyces cerevisiae
2.1.1.45 dTMP
-
 Saccharomyces cerevisiae
2.1.1.45 methotrexate
-
 Homo sapiens
2.1.1.45 methotrexate
-
 Saccharomyces cerevisiae
2.1.1.45 thymidine phosphate
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.45 0.005
-
 2'-deoxyuridylate
-
 Saccharomyces cerevisiae
2.1.1.45 0.07
-
 (-)-5,10-methylene-tetrahydropteroylglutamate
-
 Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.45 30000
-
 2 * 30000, SDS-PAGE Saccharomyces cerevisiae
2.1.1.45 65000
-
 gel filtration, velocity sedimentation electrophoresis Saccharomyces cerevisiae
2.1.1.45 68000
-
 gel filtration Saccharomyces cerevisiae
2.1.1.45 69000
-
 Ehrlich ascites carcinoma cells Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Gallus gallus
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Mus musculus
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Saccharomyces cerevisiae
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Lacticaseibacillus casei
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Homo sapiens biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Enterococcus faecalis biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism dihydrofolate + dTMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Enterococcus faecalis
-
-
-
2.1.1.45 Gallus gallus
-
 chicken
-
2.1.1.45 Homo sapiens
-
-
-
2.1.1.45 Lacticaseibacillus casei
-
-
-
2.1.1.45 Mus musculus
-
 Ehrlich ascites carcinoma cells
-
2.1.1.45 Saccharomyces cerevisiae
-
 bakers' yeast
-
2.1.1.45 Saccharomyces cerevisiae
-
 haploid strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.45
-
 Saccharomyces cerevisiae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.45 blast cell
-
 Homo sapiens
-
2.1.1.45 embryo
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.45 1.2
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Gallus gallus dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Gallus gallus dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Mus musculus dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Mus musculus dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Homo sapiens dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Saccharomyces cerevisiae dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Saccharomyces cerevisiae dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Enterococcus faecalis dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Lacticaseibacillus casei dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Lacticaseibacillus casei dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism Homo sapiens dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism Enterococcus faecalis dihydrofolate + dTMP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.45 dimer
-
 Mus musculus
2.1.1.45 dimer
-
 Homo sapiens
2.1.1.45 dimer
-
 Lacticaseibacillus casei
2.1.1.45 dimer 2 * 30000, SDS-PAGE Saccharomyces cerevisiae
2.1.1.45 monomer 1 * 69000, Ehrlich ascites carcinoma cells Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.45 6.8 7.2
-
 Saccharomyces cerevisiae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.1.1.45 5.8 8.5 half-maximal activity at either pH 5.8 or pH 8.5 Saccharomyces cerevisiae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.45 0.000004
-
 Cibacron blue
-
 Saccharomyces cerevisiae
2.1.1.45 0.000005
-
 5-fluoro-2'-deoxyuridylic acid
-
 Saccharomyces cerevisiae
2.1.1.45 0.01
-
 5-bromo-2'-deoxyuridine 5'-monophosphate
-
 Saccharomyces cerevisiae
2.1.1.45 0.015
-
 aminopterin
-
 Saccharomyces cerevisiae
2.1.1.45 0.02
-
 methotrexate
-
 Saccharomyces cerevisiae
2.1.1.45 0.02
-
 aminopterin
-
 Saccharomyces cerevisiae
2.1.1.45 0.08
-
 thymidine phosphate
-
 Saccharomyces cerevisiae
2.1.1.45 0.1
-
 dTMP
-
 Saccharomyces cerevisiae
2.1.1.45 0.12
-
 thymidine phosphate
-
 Saccharomyces cerevisiae