Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Dunlap, R.B.
    TMP synthetase from Lactobacillus casei (1978), Methods Enzymol., 51, 90-97.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.1.1.45 thawing of frozen enzyme leads to irreversible precipitation of the protein and total loss of activity, no successfully lyophilization possible Lacticaseibacillus casei

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.45 2-hydroxyethyl disulfide
-
 Lacticaseibacillus casei
2.1.1.45 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Lacticaseibacillus casei
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Lacticaseibacillus casei
2.1.1.45 cyanide
-
 Lacticaseibacillus casei
2.1.1.45 H2O2
-
 Lacticaseibacillus casei
2.1.1.45 iodoacetamide
-
 Lacticaseibacillus casei
2.1.1.45 N-ethylmaleimide
-
 Lacticaseibacillus casei
2.1.1.45 p-chloromercuribenzoate
-
 Lacticaseibacillus casei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.45 0.0051
-
 2'-deoxyuridylate in presence of 10 mM Mg2+ Lacticaseibacillus casei
2.1.1.45 0.0068
-
 2'-deoxyuridylate
-
 Lacticaseibacillus casei
2.1.1.45 0.012
-
 5,10-methylenetetrahydrofolate in presence of 10 mM Mg2+ Lacticaseibacillus casei
2.1.1.45 0.032
-
 5,10-methylenetetrahydrofolate
-
 Lacticaseibacillus casei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.45 Mg2+ in Tris-acetate buffers 10 mM enhances enzyme activity by some 30% Lacticaseibacillus casei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.45 35000
-
 2 * 35000, SDS-PAGE Lacticaseibacillus casei
2.1.1.45 70000
-
 gel filtration Lacticaseibacillus casei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Lacticaseibacillus casei
-
 dihydrofolate + dTMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Lacticaseibacillus casei
-
 var. rhamnosus, ATCC 7469
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.45
-
 Lacticaseibacillus casei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.45 2.5
-
-
 Lacticaseibacillus casei

Storage Stability

EC Number Storage Stability Organism
2.1.1.45 0-5°C, enzyme is stable over a period of months in presence of 10 mM 2-mercaptoethanol in capped vessels layered with argon Lacticaseibacillus casei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Lacticaseibacillus casei dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Lacticaseibacillus casei dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + uridine 5'-phosphate
-
 Lacticaseibacillus casei dihydrofolate + TMP
-
 r

Subunits

EC Number Subunits Comment Organism
2.1.1.45 dimer 2 * 35000, SDS-PAGE Lacticaseibacillus casei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.45 5.9
-
 UMP as substrate Lacticaseibacillus casei
2.1.1.45 6.5 6.8
-
 Lacticaseibacillus casei

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.45 (+)-methylenetetrahydrofolate
-
 Lacticaseibacillus casei