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Literature summary extracted from

  • Slavik, K.; Rode, W.; Slavikova, V.
    Purification of thymidylate synthetase from enzyme-poor sources by affinity chromatography (1976), Biochemistry, 15, 4222-4227.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.45 tetrahydromethotrexate
-
 Bos taurus
2.1.1.45 tetrahydromethotrexate
-
 Escherichia coli
2.1.1.45 tetrahydromethotrexate
-
 Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Bos taurus
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Mus musculus key function in DNA synthesis, repair and cell division dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Escherichia coli key function in DNA synthesis, repair and cell division dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Escherichia coli B / ATCC 11303 key function in DNA synthesis, repair and cell division dihydrofolate + dTMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Bos taurus
-
 calf
-
2.1.1.45 Escherichia coli
-
-
-
2.1.1.45 Escherichia coli B / ATCC 11303
-
-
-
2.1.1.45 Mus musculus
-
 Gardner lymphosarcoma
-
2.1.1.45 Mus musculus
-
 mouse, sarcoma 180
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.45
-
 Mus musculus
2.1.1.45
-
 Escherichia coli
2.1.1.45
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.45 thymus
-
 Mus musculus
-
2.1.1.45 thymus
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.45 0.0156
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.1.1.45 0°C, enzyme can be maintained in its active form in presence of saccharose or glycerol for several days Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Mus musculus dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Escherichia coli dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Bos taurus dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Bos taurus dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP key function in DNA synthesis, repair and cell division Mus musculus dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP key function in DNA synthesis, repair and cell division Escherichia coli dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Escherichia coli B / ATCC 11303 dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP key function in DNA synthesis, repair and cell division Escherichia coli B / ATCC 11303 dihydrofolate + dTMP
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.45 0.00012
-
 tetrahydromethotrexate
-
 Escherichia coli