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Literature summary extracted from

  • Poulton, J.E.; Hahlbrock, K.; Grisebach, H.
    O-Methylation of flavonoid substrates by a partially purified enzyme from soybean cell suspension cultures (1977), Arch. Biochem. Biophys., 180, 543-549.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.42 EDTA
-
 Glycine max
2.1.1.42 EDTA
-
 Petroselinum crispum
2.1.1.42 S-adenosyl-L-homocysteine inhibits the methylation of luteolin by S-adenosylmethionine Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.42 0.011
-
 S-adenosyl-L-methionine with luteolin as substrate Glycine max
2.1.1.42 0.016
-
 5,7,3',4'-tetrahydroxyflavone
-
 Glycine max
2.1.1.42 0.028
-
 5,7,3'-trihydroxyflavone 7-O-glucoside
-
 Glycine max
2.1.1.42 0.035
-
 6,7-dihydroxy-3'-methoxyisoflavone
-
 Glycine max
2.1.1.42 0.075
-
 5,7,3',4'-tetrahydroxyflavanone
-
 Glycine max
2.1.1.42 0.227
-
 5-hydroxy-3-methoxy-4-hydroxycinnamic acid
-
 Glycine max
2.1.1.42 0.435
-
 dihydroquercetin
-
 Glycine max

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.42 Mg2+ required for activity Glycine max
2.1.1.42 Mg2+ required for activity Petroselinum crispum

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.42 Glycine max
-
 soybean, L. var. Mandarin
-
2.1.1.42 Petroselinum crispum
-
 parsley
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.42
-
 Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.42 cell suspension culture
-
 Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.42 0.0029
-
-
 Glycine max

Storage Stability

EC Number Storage Stability Organism
2.1.1.42 -20°C, stored in 10% glycerol for 2 months, no singnificant loss in enzyme activity Glycine max
2.1.1.42 4°C, highly unstable, stored with Triton X-100, bovine serum albumin, ethylene glycol, dimethyl sulfoxide, 2-mercaptoethanol, dithiothreitol or ethylene glycol monoethylether, loses over 90% of its activity within 94 h Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.42 additional information
-
 Petroselinum crispum ?
-
 ?
2.1.1.42 additional information methylation proceedes only in the meta-position, enzyme is unable to catalyze the methylation of p-coumaric acid, m-coumaric acid, ferulic acid, isoferulic acid, sinapic acid, apigenin and naringenin Glycine max ?
-
 ?
2.1.1.42 additional information isoflavones biochanin A and daidzein do not serve as substrates Glycine max ?
-
 ?
2.1.1.42 S-adenosyl-L-homocysteine + 6,7-dihydroxy-3'-methoxyisoflavone
-
 Glycine max ?
-
 ?
2.1.1.42 S-adenosyl-L-methionine + 3,3',4',5,7-pentahydroxy flavone quercetin Glycine max S-adenosyl-L-homocysteine + isorhamnetin
-
 ?
2.1.1.42 S-adenosyl-L-methionine + 4,5-dihydroxy-3-methoxycinnamate 5-hydroxy ferulic acid Glycine max S-adenosyl-L-homocysteine + 4-hydroxy-3,5-dimethoxycinnamate
-
 ?
2.1.1.42 S-adenosyl-L-methionine + 5,7,3',4'-tetrahydroxyflavanone eriodictyol Glycine max S-adenosyl-L-homocysteine + 5,7,4'-trihydroxy-3'-methoxyflavanone
-
 ?
2.1.1.42 S-adenosyl-L-methionine + 5,7,3',4'-tetrahydroxyflavanone eriodictyol Petroselinum crispum S-adenosyl-L-homocysteine + 5,7,4'-trihydroxy-3'-methoxyflavanone
-
 ?
2.1.1.42 S-adenosyl-L-methionine + 5,7,3',4'-tetrahydroxyflavone luteolin Glycine max S-adenosyl-L-homocysteine + 5,7,4'-trihydroxy-3'-methoxyflavone
-
 ?
2.1.1.42 S-adenosyl-L-methionine + 5,7,3',4'-tetrahydroxyflavone luteolin Petroselinum crispum S-adenosyl-L-homocysteine + 5,7,4'-trihydroxy-3'-methoxyflavone
-
 ?
2.1.1.42 S-adenosyl-L-methionine + 5,7,3'-trihydroxyflavone 7-O-glucoside
-
 Glycine max S-adenosyl-L-homocysteine + 5,7,4'-trihydroxy-3'-methoxyflavone 7-O-glucoside chrysoeriol-7-O-glucoside ?
2.1.1.42 S-adenosyl-L-methionine + 5,7,3'-trihydroxyflavone 7-O-glucoside
-
 Petroselinum crispum S-adenosyl-L-homocysteine + 5,7,4'-trihydroxy-3'-methoxyflavone 7-O-glucoside chrysoeriol-7-O-glucoside ?
2.1.1.42 S-adenosyl-L-methionine + dihydro-3,3',4',5,7-pentahydroxy flavone dihydroquercetin Glycine max S-adenosyl-L-homocysteine + dihydroisorhamnetin
-
 ?
2.1.1.42 S-adenosyl-L-methionine + quercetin 3-O-glucoside poor substrate Glycine max S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.42 S-adenosyl-L-methionine + rutin poor substrate Glycine max S-adenosyl-L-homocysteine + ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.42 8.6 8.9 methylation of quercetin Glycine max

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.42 6 10
-
 Glycine max