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Literature summary extracted from

  • Shi, J.; Gonzales, R.A.; Bhattacharyya, M.K.
    Identification and characterization of an S-adenosyl-L-methionine:DELTA24-sterol-C-methyltransferase cDNA from soybean (1996), J. Biol. Chem., 271, 9384-9389.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.142 expression as FLAG-fusion protein in Escherichia coli, amino acid sequence analysis Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.142 cycloartenol + S-adenosyl-L-methionine Glycine max
-
 24-methylenecycloartanol + S-adenosyl-L-homocysteine
-
 ?
2.1.1.142 cycloartenol + S-adenosyl-L-methionine Glycine max rate-limiting initial step in the conversion of phytosterol 24-methylenecycloartanol + S-adenosyl-L-homocysteine
-
 ?
2.1.1.142 zymosterol + S-adenosyl-L-methionine Glycine max
-
 fecosterol + S-adenosyl-L-homocysteine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.142 Glycine max
-
 gene SMT1
-
2.1.1.142 Glycine max Q43445
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.142 flower
-
 Glycine max
-
2.1.1.142 hypocotyl etiolated Glycine max
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.142 cycloartenol + S-adenosyl-L-methionine
-
 Glycine max 24-methylenecycloartanol + S-adenosyl-L-homocysteine
-
 ?
2.1.1.142 cycloartenol + S-adenosyl-L-methionine rate-limiting initial step in the conversion of phytosterol Glycine max 24-methylenecycloartanol + S-adenosyl-L-homocysteine
-
 ?
2.1.1.142 zymosterol + S-adenosyl-L-methionine
-
 Glycine max fecosterol + S-adenosyl-L-homocysteine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.142 30
-
 assay at Glycine max