Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wagner, C.; Lusty, S.M.; Kung, H.; Rogers, N.L.
    Preparation and properties of trimethylsulfonium-tetrahydrofolate methyltransferase (1967), J. Biol. Chem., 242, 1287-1293.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.19 2-mercaptoethanol reducing agent required for activity Pseudomonas sp.
2.1.1.19 ascorbate can replace 2-mercaptoethanol, best reducing agent for reaction assay Pseudomonas sp.
2.1.1.19 homocysteine can replace 2-mercaptoethanol Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.19 2-mercaptoethanol inhibitory in excess Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.19 1
-
 tetrahydrofolate
-
 Pseudomonas sp.
2.1.1.19 5.8
-
 Trimethylsulfonium
-
 Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.19 100000
-
 sucrose density gradient centrifugation Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.19 Pseudomonas sp.
-
 atypical pseudomonad with two lateral, subpolar flagellae
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.19
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.19 0.007
-
 purified enzyme Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
2.1.1.19 -20°C, 80% activity retained after 4 months Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.19 additional information S-adenosylmethionine and dimethyl-beta-propiothetin are not accepted as methyl donors Pseudomonas sp. ?
-
 ?
2.1.1.19 trimethylsulfonium + tetrahydrofolate
-
 Pseudomonas sp. dimethylsulfide + 5-methyltetrahydrofolate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.19 7.8
-
 broad optimum Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.19 tetrahydrofolate
-
 Pseudomonas sp.