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Literature summary extracted from

  • Gang, D.R.; Lavid, N.; Zubieta, C.; Chen, F.; Beuerle, T.; Lewinsohn, E.; Noel, J.P.; Pichersky, E.
    Characterization of phenylpropene O-methyltransferases from sweet basil: facile change of substrate specificity and convergent evolution within a plant O-methyltransferase family (2002), Plant Cell, 14, 505-519.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.146
-
 Ocimum basilicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.146 0.003
-
 eugenol
-
 Ocimum basilicum
2.1.1.146 0.005
-
 S-adenosyl-L-methionine
-
 Ocimum basilicum
2.1.1.146 0.007
-
 chavicol
-
 Ocimum basilicum
2.1.1.146 0.01
-
 trans-isoeugenol
-
 Ocimum basilicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.146 40000
-
 2 * 40000, SDS-PAGE Ocimum basilicum
2.1.1.146 81000
-
 gel filtration Ocimum basilicum

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.146 Ocimum basilicum Q93WU2 variety EMX-1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.146 partial Ocimum basilicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.146 leaf young Ocimum basilicum
-
2.1.1.146 trichome
-
 Ocimum basilicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.146 S-adenosyl-L-methionine + chavicol 24% of the activity with eugenol Ocimum basilicum S-adenosyl-L-homocysteine + estragol
-
 ?
2.1.1.146 S-adenosyl-L-methionine + eugenol
-
 Ocimum basilicum S-adenosyl-L-homocysteine + methyleugenol
-
 ?
2.1.1.146 S-adenosyl-L-methionine + guaiacol 29.9% of the activity with eugenol Ocimum basilicum S-adenosyl-L-homocysteine + 1,2-dimethoxybenzene
-
 ?
2.1.1.146 S-adenosyl-L-methionine + isoeugenol 26% of the activity with eugenol Ocimum basilicum S-adenosyl-L-homocysteine + isomethyleugenol
-
 ?
2.1.1.146 S-adenosyl-L-methionine + trans-isoeugenol
-
 Ocimum basilicum S-adenosyl-L-homocysteine + (E)-2',3'-dimethoxy-1-propenylbenzene
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.146 dimer 2 * 40000, SDS-PAGE Ocimum basilicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.146 0.00087
-
 chavicol
-
 Ocimum basilicum
2.1.1.146 0.0013
-
 t-isoeugenol
-
 Ocimum basilicum
2.1.1.146 0.0031
-
 eugenol
-
 Ocimum basilicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.146 7 8
-
 Ocimum basilicum