BRENDA - Enzyme Database

Characterization of phenylpropene O-methyltransferases from sweet basil: facile change of substrate specificity and convergent evolution within a plant O-methyltransferase family

Gang, D.R.; Lavid, N.; Zubieta, C.; Chen, F.; Beuerle, T.; Lewinsohn, E.; Noel, J.P.; Pichersky, E.; Plant Cell 14, 505-519 (2002)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
2.1.1.146
-
Ocimum basilicum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.146
0.003
-
eugenol
-
Ocimum basilicum
2.1.1.146
0.005
-
S-adenosyl-L-methionine
-
Ocimum basilicum
2.1.1.146
0.007
-
chavicol
-
Ocimum basilicum
2.1.1.146
0.01
-
trans-isoeugenol
-
Ocimum basilicum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.146
40000
-
2 * 40000, SDS-PAGE
Ocimum basilicum
2.1.1.146
81000
-
gel filtration
Ocimum basilicum
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.1.1.146
Ocimum basilicum
Q93WU2
variety EMX-1
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
2.1.1.146
partial
Ocimum basilicum
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.146
leaf
young
Ocimum basilicum
-
2.1.1.146
trichome
-
Ocimum basilicum
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.1.1.146
S-adenosyl-L-methionine + chavicol
24% of the activity with eugenol
485155
Ocimum basilicum
S-adenosyl-L-homocysteine + estragol
-
-
-
?
2.1.1.146
S-adenosyl-L-methionine + eugenol
-
485155
Ocimum basilicum
S-adenosyl-L-homocysteine + methyleugenol
-
-
-
?
2.1.1.146
S-adenosyl-L-methionine + guaiacol
29.9% of the activity with eugenol
485155
Ocimum basilicum
S-adenosyl-L-homocysteine + 1,2-dimethoxybenzene
-
-
-
?
2.1.1.146
S-adenosyl-L-methionine + isoeugenol
26% of the activity with eugenol
485155
Ocimum basilicum
S-adenosyl-L-homocysteine + isomethyleugenol
-
485155
Ocimum basilicum
?
2.1.1.146
S-adenosyl-L-methionine + trans-isoeugenol
-
485155
Ocimum basilicum
S-adenosyl-L-homocysteine + (E)-2',3'-dimethoxy-1-propenylbenzene
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.1.1.146
dimer
2 * 40000, SDS-PAGE
Ocimum basilicum
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.1.1.146
0.00087
-
chavicol
-
Ocimum basilicum
2.1.1.146
0.0013
-
t-isoeugenol
-
Ocimum basilicum
2.1.1.146
0.0031
-
eugenol
-
Ocimum basilicum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.146
7
8
-
Ocimum basilicum
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.146
-
Ocimum basilicum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.146
0.003
-
eugenol
-
Ocimum basilicum
2.1.1.146
0.005
-
S-adenosyl-L-methionine
-
Ocimum basilicum
2.1.1.146
0.007
-
chavicol
-
Ocimum basilicum
2.1.1.146
0.01
-
trans-isoeugenol
-
Ocimum basilicum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.146
40000
-
2 * 40000, SDS-PAGE
Ocimum basilicum
2.1.1.146
81000
-
gel filtration
Ocimum basilicum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.146
partial
Ocimum basilicum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.146
leaf
young
Ocimum basilicum
-
2.1.1.146
trichome
-
Ocimum basilicum
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.1.1.146
S-adenosyl-L-methionine + chavicol
24% of the activity with eugenol
485155
Ocimum basilicum
S-adenosyl-L-homocysteine + estragol
-
-
-
?
2.1.1.146
S-adenosyl-L-methionine + eugenol
-
485155
Ocimum basilicum
S-adenosyl-L-homocysteine + methyleugenol
-
-
-
?
2.1.1.146
S-adenosyl-L-methionine + guaiacol
29.9% of the activity with eugenol
485155
Ocimum basilicum
S-adenosyl-L-homocysteine + 1,2-dimethoxybenzene
-
-
-
?
2.1.1.146
S-adenosyl-L-methionine + isoeugenol
26% of the activity with eugenol
485155
Ocimum basilicum
S-adenosyl-L-homocysteine + isomethyleugenol
-
485155
Ocimum basilicum
?
2.1.1.146
S-adenosyl-L-methionine + trans-isoeugenol
-
485155
Ocimum basilicum
S-adenosyl-L-homocysteine + (E)-2',3'-dimethoxy-1-propenylbenzene
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.1.1.146
dimer
2 * 40000, SDS-PAGE
Ocimum basilicum
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.1.1.146
0.00087
-
chavicol
-
Ocimum basilicum
2.1.1.146
0.0013
-
t-isoeugenol
-
Ocimum basilicum
2.1.1.146
0.0031
-
eugenol
-
Ocimum basilicum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.146
7
8
-
Ocimum basilicum