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Literature summary extracted from

  • James, F.; Nolte, K.D.; Hanson, A.D.
    Purification and properties of S-adenosyl-L-methionine:L-methionine S-methyltransferase from Wollastonia biflora leaves (1995), J. Biol. Chem., 270, 22344-22350.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.12 3-(methylthio)propanol
-
 Wollastonia biflora
2.1.1.12 3-(methylthio)propionate
-
 Wollastonia biflora
2.1.1.12 3-(methylthio)propionate methyl ester
-
 Wollastonia biflora
2.1.1.12 3-(methylthio)propylamine
-
 Wollastonia biflora
2.1.1.12 3-dimethylsulfoniopropionaldehyde
-
 Wollastonia biflora
2.1.1.12 D-Met
-
 Wollastonia biflora
2.1.1.12 DL-2-hydroxy-4-(methylthio)butyrate
-
 Wollastonia biflora
2.1.1.12 Gly-Met
-
 Wollastonia biflora
2.1.1.12 L-ethionine
-
 Wollastonia biflora
2.1.1.12 L-Met ethyl ester
-
 Wollastonia biflora
2.1.1.12 L-Met methyl ester
-
 Wollastonia biflora
2.1.1.12 L-Met p-nitroanilide
-
 Wollastonia biflora
2.1.1.12 L-Methioninamide
-
 Wollastonia biflora
2.1.1.12 L-methioninol
-
 Wollastonia biflora
2.1.1.12 N-2,4-dinitrophenyl-DL-Met
-
 Wollastonia biflora
2.1.1.12 N-formyl-L-Met
-
 Wollastonia biflora
2.1.1.12 NEM 0.5 mM, 99% inhibition Wollastonia biflora
2.1.1.12 p-hydroxymercuribenzoate 0.1 mM; 97% inhibition Wollastonia biflora
2.1.1.12 S-adenosyl-L-homocysteine
-
 Wollastonia biflora
2.1.1.12 S-methyl-L-methionine
-
 Wollastonia biflora
2.1.1.12 seleno-L-methionine
-
 Wollastonia biflora

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.12 0.034
-
 S-adenosyl-L-methionine
-
 Wollastonia biflora
2.1.1.12 0.14
-
 L-Met
-
 Wollastonia biflora

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.12 115000
-
 4 * 115000, SDS-PAGE Wollastonia biflora
2.1.1.12 450000
-
 gel filtration Wollastonia biflora

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.12 Brassica oleracea
-
-
-
2.1.1.12 Lactuca sativa
-
-
-
2.1.1.12 Trifolium repens
-
-
-
2.1.1.12 Wollastonia biflora
-
-
-
2.1.1.12 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.12
-
 Wollastonia biflora

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.1.12 S-adenosyl-L-methionine + L-methionine + H+ = S-adenosyl-L-homocysteine + S-methyl-L-methionine ordered bi bi mechanism with S-adenosylmethionine the first reactant to bind and S-adenosylhomocysteine the last product to be released Wollastonia biflora
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.12 leaf
-
 Wollastonia biflora
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.12 additional information
-
-
 Wollastonia biflora

Storage Stability

EC Number Storage Stability Organism
2.1.1.12 -20 C, complete inactivation after flash freezing in liquid N2 Wollastonia biflora

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.12 S-adenosyl-L-methionine + L-methionine
-
 Zea mays S-adenosyl-L-homocysteine + S-methyl-L-methionine
-
 ?
2.1.1.12 S-adenosyl-L-methionine + L-methionine
-
 Lactuca sativa S-adenosyl-L-homocysteine + S-methyl-L-methionine
-
 ?
2.1.1.12 S-adenosyl-L-methionine + L-methionine
-
 Brassica oleracea S-adenosyl-L-homocysteine + S-methyl-L-methionine
-
 ?
2.1.1.12 S-adenosyl-L-methionine + L-methionine
-
 Trifolium repens S-adenosyl-L-homocysteine + S-methyl-L-methionine
-
 ?
2.1.1.12 S-adenosyl-L-methionine + L-methionine
-
 Wollastonia biflora S-adenosyl-L-homocysteine + S-methyl-L-methionine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.12 tetramer 4 * 115000, SDS-PAGE Wollastonia biflora

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.12 4
-
 complete inactivation after 1 week Wollastonia biflora
2.1.1.12 25
-
 complete inactivation after 24 h Wollastonia biflora

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.12 7.2
-
-
 Wollastonia biflora

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.12 5.8 9 about 50-55% of optimal activity at pH 5.8 and 9.0, only 1% of optimal activity at pH 5.0 Wollastonia biflora

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.12 0.013
-
 S-adenosyl-L-homocysteine
-
 Wollastonia biflora
2.1.1.12 0.226
-
 S-methyl-L-methionine
-
 Wollastonia biflora