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Literature summary extracted from

  • Yeh, W.K.; Bauer, N.J.; Dotzlaf, J.E.
    High-performance liquid chromatographic assay for S-adenosyl-L-methionine: macrocin O-methyltransferase (1984), J. Chromatogr., 288, 157-165.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.101 Relomycin
-
 Streptomyces fradiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.101 0.029
-
 S-adenosyl-L-methionine
-
 Streptomyces fradiae
2.1.1.101 0.033
-
 macrocin
-
 Streptomyces fradiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.101 Co2+ stimulated by cobalt chloride Streptomyces fradiae
2.1.1.101 Fe2+ stimulated by iron (II) sulfate Streptomyces fradiae
2.1.1.101 Mg2+ stimulated by magnesium sulfate Streptomyces fradiae
2.1.1.101 Mn2+ stimulated by manganese sulfate Streptomyces fradiae
2.1.1.101 Zn2+ stimulated by zinc sulfate Streptomyces fradiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.101 S-adenosyl-L-methionine + macrocin Streptomyces fradiae terminal and main rate-limiting step in tylosin biosynthesis S-adenosylhomocysteine + tylosin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.101 Streptomyces fradiae
-
-
-

Storage Stability

EC Number Storage Stability Organism
2.1.1.101 4°C, enzyme is unstable, about 30% activity remains after 3 h Streptomyces fradiae
2.1.1.101 4°C, very stable for 3 h in presence of 10% ethanol Streptomyces fradiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.101 S-adenosyl-L-methionine + macrocin
-
 Streptomyces fradiae S-adenosyl-L-homocysteine + tylosin
-
 ?
2.1.1.101 S-adenosyl-L-methionine + macrocin terminal and main rate-limiting step in tylosin biosynthesis Streptomyces fradiae S-adenosylhomocysteine + tylosin
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.101 7.8
-
-
 Streptomyces fradiae