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Literature summary extracted from

  • Nare, B.; Luba, J.; Hardy, L.W.; Beverley, S.
    New approaches to Leishmania chemotherapy: pteridine reductase 1 (PTR1) as a target and modulator of antifolate sensitivity (1997), Parasitology, 114, 101-110.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.5.1.33 pharmacology successful antifolate chemotherapy in Leishmania will have to target simultaneously both pterine reductase 1 and dihydrofolate reductase-thymidylate synthase Leishmania major

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.33 dihydrobiopterin substrate inhibition Leishmania major
1.5.1.33 dihydrofolate substrate inhibition Leishmania major
1.5.1.33 additional information
-
Leishmania major

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.33 0.012
-
NADPH NADPH Leishmania major

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.33 30000
-
4 * 30000 Leishmania major

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.33 Leishmania major
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.5.1.33 5,6,7,8-tetrahydrobiopterin + 2 NADP+ = biopterin + 2 NADPH + 2 H+ ordered sequential reaction mechanism Leishmania major

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.33 Biopterin + NADPH
-
Leishmania major 5,6,7,8-Tetrahydrobiopterin + NADP+
-
?
1.5.1.33 Dihydrobiopterin + NADPH
-
Leishmania major ?
-
?
1.5.1.33 Dihydrofolate + NADPH
-
Leishmania major 5,6,7,8-Tetrahydrofolate + NADP+
-
?

Subunits

EC Number Subunits Comment Organism
1.5.1.33 tetramer 4 * 30000 Leishmania major

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.33 4.7
-
biopterin as substrate Leishmania major
1.5.1.33 6
-
folate as substrate Leishmania major