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Literature summary extracted from

  • Abeles, R.H.
    D-Proline reductase (Clostridium sticklandii) (1971), Methods Enzymol., 17, 317-321.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.21.4.1 pyruvate covalently bound Acetoanaerobium sticklandii

General Stability

EC Number General Stability Organism
1.21.4.1 stability is greatly enhanced by polyvalent ions such as SO42- or HPO42- Acetoanaerobium sticklandii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.21.4.1 hydroxylamine
-
 Acetoanaerobium sticklandii
1.21.4.1 iodoacetamide
-
 Acetoanaerobium sticklandii
1.21.4.1 iodoacetate
-
 Acetoanaerobium sticklandii
1.21.4.1 NaBH4 irreversible inactivation Acetoanaerobium sticklandii
1.21.4.1 phenylhydrazine
-
 Acetoanaerobium sticklandii

Organism

EC Number Organism UniProt Comment Textmining
1.21.4.1 Acetoanaerobium sticklandii
-
 strain ATCC12662 identical with strain DSM 519 T
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.21.4.1
-
 Acetoanaerobium sticklandii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.21.4.1 2.71
-
-
 Acetoanaerobium sticklandii

Storage Stability

EC Number Storage Stability Organism
1.21.4.1 0-5°C purified enzyme stable for at least 1 month Acetoanaerobium sticklandii
1.21.4.1 0°C frozen in solution retains constant enzyme activity for at least a week Acetoanaerobium sticklandii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.4.1 D-proline + dithiol (reduced)
-
 Acetoanaerobium sticklandii 5-aminopentanoic acid + oxidized dithiol
-
 ir

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.21.4.1 8.6
-
 maximum stability Acetoanaerobium sticklandii