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Literature summary extracted from

  • Couderc, R.; Baratti, J.
    Oxidation of methanol by the yeast, Pichia pastoris. Purification and properties of the alcohol oxidase (1980), Agric. Biol. Chem., 44, 2279-2289.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.13 H2O2
-
 Komagataella pastoris
1.1.3.13 H2O2
-
 Ogataea angusta

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.13 0.4
-
 O2 methanol concentration 1 mM Komagataella pastoris
1.1.3.13 0.7
-
 O2 methanol concentration 10 mM Komagataella pastoris
1.1.3.13 1
-
 ethanol
-
 Komagataella pastoris
1.1.3.13 1
-
 O2 methanol concentration 100 mM Komagataella pastoris
1.1.3.13 1.4
-
 methanol oxygen concentration 0.19 mM Komagataella pastoris
1.1.3.13 3.1
-
 methanol oxygen concentration 0.93 mM Komagataella pastoris

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.3.13 cytoplasm
-
 Komagataella pastoris 5737
-
1.1.3.13 cytoplasm
-
 Ogataea angusta 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.13 80000
-
 8 * 80000, SDS-PAGE Komagataella pastoris
1.1.3.13 675000
-
 analytical ultracentrifugation, gel filtration Komagataella pastoris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.13 methanol + O2 Komagataella pastoris key enzyme of methanol metabolism formaldehyde + H2O2
-
 ?
1.1.3.13 methanol + O2 Ogataea angusta key enzyme of methanol metabolism formaldehyde + H2O2
-
 ?
1.1.3.13 methanol + O2 Komagataella pastoris IFP 206 key enzyme of methanol metabolism formaldehyde + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.13 Komagataella pastoris
-
-
-
1.1.3.13 Komagataella pastoris IFP 206
-
-
-
1.1.3.13 Ogataea angusta
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.13
-
 Komagataella pastoris

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.13 11.9
-
-
 Komagataella pastoris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.13 ethanol + O2
-
 Ogataea angusta acetaldehyde + H2O2
-
 ?
1.1.3.13 ethanol + O2 82% of the activity with methanol Komagataella pastoris acetaldehyde + H2O2
-
 ?
1.1.3.13 ethanol + O2 82% of the activity with methanol Komagataella pastoris IFP 206 acetaldehyde + H2O2
-
 ?
1.1.3.13 isobutanol + O2 1.2% of the activity with methanol Komagataella pastoris isobutyraldehyde + H2O2
-
 ?
1.1.3.13 methanol + O2
-
 Komagataella pastoris formaldehyde + H2O2
-
 ?
1.1.3.13 methanol + O2
-
 Ogataea angusta formaldehyde + H2O2
-
 ?
1.1.3.13 methanol + O2 key enzyme of methanol metabolism Komagataella pastoris formaldehyde + H2O2
-
 ?
1.1.3.13 methanol + O2 key enzyme of methanol metabolism Ogataea angusta formaldehyde + H2O2
-
 ?
1.1.3.13 methanol + O2
-
 Komagataella pastoris IFP 206 formaldehyde + H2O2
-
 ?
1.1.3.13 methanol + O2 key enzyme of methanol metabolism Komagataella pastoris IFP 206 formaldehyde + H2O2
-
 ?
1.1.3.13 n-butanol + O2
-
 Ogataea angusta n-butanal + H2O2
-
 ?
1.1.3.13 n-butanol + O2 20% of the activity with methanol Komagataella pastoris n-butanal + H2O2
-
 ?
1.1.3.13 n-propanol + O2
-
 Ogataea angusta n-propanal + H2O2
-
 ?
1.1.3.13 n-propanol + O2 43% of the activity with methanol Komagataella pastoris n-propanal + H2O2
-
 ?
1.1.3.13 n-propanol + O2 43% of the activity with methanol Komagataella pastoris IFP 206 n-propanal + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.13 octamer
-
 Ogataea angusta
1.1.3.13 octamer 8 * 80000, SDS-PAGE Komagataella pastoris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.13 37
-
-
 Komagataella pastoris

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.3.13 18 45 half-maximal activity at 18°C and 45°C Komagataella pastoris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.13 7.5
-
-
 Komagataella pastoris
1.1.3.13 8.5
-
-
 Ogataea angusta

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.13 6.5 8.3 about half-maximal activity at pH 6.5 and 8.3 Komagataella pastoris
1.1.3.13 6.7 9.8 about half-maximal activity at pH 6.7 and 9.8 Ogataea angusta

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.13 7 8 stable Komagataella pastoris
1.1.3.13 8 10 stable Ogataea angusta
1.1.3.13 9
-
 and above, inactivation Komagataella pastoris

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.13 FAD 1 mol FAD per mol of subunit Komagataella pastoris
1.1.3.13 FAD 1 mol FAD per mol of subunit Ogataea angusta