BRENDA - Enzyme Database show

Oxidation of methanol by the yeast, Pichia pastoris. Purification and properties of the alcohol oxidase

Couderc, R.; Baratti, J.; Agric. Biol. Chem. 44, 2279-2289 (1980)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.3.13
H2O2
-
Ogataea angusta
1.1.3.13
H2O2
-
Komagataella pastoris
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.3.13
0.4
-
O2
methanol concentration 1 mM
Komagataella pastoris
1.1.3.13
0.7
-
O2
methanol concentration 10 mM
Komagataella pastoris
1.1.3.13
1
-
ethanol
-
Komagataella pastoris
1.1.3.13
1
-
O2
methanol concentration 100 mM
Komagataella pastoris
1.1.3.13
1.4
-
methanol
oxygen concentration 0.19 mM
Komagataella pastoris
1.1.3.13
3.1
-
methanol
oxygen concentration 0.93 mM
Komagataella pastoris
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.3.13
cytoplasm
-
Komagataella pastoris
5737
-
1.1.3.13
cytoplasm
-
Ogataea angusta
5737
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.3.13
80000
-
8 * 80000, SDS-PAGE
Komagataella pastoris
1.1.3.13
675000
-
analytical ultracentrifugation, gel filtration
Komagataella pastoris
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.3.13
methanol + O2
Komagataella pastoris
key enzyme of methanol metabolism
formaldehyde + H2O2
-
-
?
1.1.3.13
methanol + O2
Ogataea angusta
key enzyme of methanol metabolism
formaldehyde + H2O2
-
-
?
1.1.3.13
methanol + O2
Komagataella pastoris IFP 206
key enzyme of methanol metabolism
formaldehyde + H2O2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.3.13
Komagataella pastoris
-
-
-
1.1.3.13
Komagataella pastoris IFP 206
-
-
-
1.1.3.13
Ogataea angusta
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.3.13
-
Komagataella pastoris
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.3.13
11.9
-
-
Komagataella pastoris
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.3.13
ethanol + O2
-
484910
Ogataea angusta
acetaldehyde + H2O2
-
-
-
?
1.1.3.13
ethanol + O2
82% of the activity with methanol
484910
Komagataella pastoris
acetaldehyde + H2O2
-
-
-
?
1.1.3.13
ethanol + O2
82% of the activity with methanol
484910
Komagataella pastoris IFP 206
acetaldehyde + H2O2
-
-
-
?
1.1.3.13
isobutanol + O2
1.2% of the activity with methanol
484910
Komagataella pastoris
isobutyraldehyde + H2O2
-
-
-
?
1.1.3.13
methanol + O2
-
484910
Komagataella pastoris
formaldehyde + H2O2
-
484910
Komagataella pastoris
?
1.1.3.13
methanol + O2
-
484910
Ogataea angusta
formaldehyde + H2O2
-
484910
Ogataea angusta
?
1.1.3.13
methanol + O2
key enzyme of methanol metabolism
484910
Komagataella pastoris
formaldehyde + H2O2
-
-
-
?
1.1.3.13
methanol + O2
key enzyme of methanol metabolism
484910
Ogataea angusta
formaldehyde + H2O2
-
-
-
?
1.1.3.13
methanol + O2
-
484910
Komagataella pastoris IFP 206
formaldehyde + H2O2
-
484910
Komagataella pastoris IFP 206
?
1.1.3.13
methanol + O2
key enzyme of methanol metabolism
484910
Komagataella pastoris IFP 206
formaldehyde + H2O2
-
-
-
?
1.1.3.13
n-butanol + O2
-
484910
Ogataea angusta
n-butanal + H2O2
-
-
-
?
1.1.3.13
n-butanol + O2
20% of the activity with methanol
484910
Komagataella pastoris
n-butanal + H2O2
-
-
-
?
1.1.3.13
n-propanol + O2
-
484910
Ogataea angusta
n-propanal + H2O2
-
-
-
?
1.1.3.13
n-propanol + O2
43% of the activity with methanol
484910
Komagataella pastoris
n-propanal + H2O2
-
-
-
?
1.1.3.13
n-propanol + O2
43% of the activity with methanol
484910
Komagataella pastoris IFP 206
n-propanal + H2O2
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.3.13
octamer
8 * 80000, SDS-PAGE
Komagataella pastoris
1.1.3.13
octamer
-
Ogataea angusta
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.3.13
37
-
-
Komagataella pastoris
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.1.3.13
18
45
half-maximal activity at 18°C and 45°C
Komagataella pastoris
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.3.13
7.5
-
-
Komagataella pastoris
1.1.3.13
8.5
-
-
Ogataea angusta
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.3.13
6.5
8.3
about half-maximal activity at pH 6.5 and 8.3
Komagataella pastoris
1.1.3.13
6.7
9.8
about half-maximal activity at pH 6.7 and 9.8
Ogataea angusta
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.1.3.13
7
8
stable
Komagataella pastoris
1.1.3.13
8
10
stable
Ogataea angusta
1.1.3.13
9
-
and above, inactivation
Komagataella pastoris
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.3.13
FAD
1 mol FAD per mol of subunit
Komagataella pastoris
1.1.3.13
FAD
1 mol FAD per mol of subunit
Ogataea angusta
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.3.13
FAD
1 mol FAD per mol of subunit
Komagataella pastoris
1.1.3.13
FAD
1 mol FAD per mol of subunit
Ogataea angusta
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.3.13
H2O2
-
Ogataea angusta
1.1.3.13
H2O2
-
Komagataella pastoris
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.3.13
0.4
-
O2
methanol concentration 1 mM
Komagataella pastoris
1.1.3.13
0.7
-
O2
methanol concentration 10 mM
Komagataella pastoris
1.1.3.13
1
-
ethanol
-
Komagataella pastoris
1.1.3.13
1
-
O2
methanol concentration 100 mM
Komagataella pastoris
1.1.3.13
1.4
-
methanol
oxygen concentration 0.19 mM
Komagataella pastoris
1.1.3.13
3.1
-
methanol
oxygen concentration 0.93 mM
Komagataella pastoris
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.3.13
cytoplasm
-
Komagataella pastoris
5737
-
1.1.3.13
cytoplasm
-
Ogataea angusta
5737
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.3.13
80000
-
8 * 80000, SDS-PAGE
Komagataella pastoris
1.1.3.13
675000
-
analytical ultracentrifugation, gel filtration
Komagataella pastoris
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.3.13
methanol + O2
Komagataella pastoris
key enzyme of methanol metabolism
formaldehyde + H2O2
-
-
?
1.1.3.13
methanol + O2
Ogataea angusta
key enzyme of methanol metabolism
formaldehyde + H2O2
-
-
?
1.1.3.13
methanol + O2
Komagataella pastoris IFP 206
key enzyme of methanol metabolism
formaldehyde + H2O2
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.3.13
-
Komagataella pastoris
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.3.13
11.9
-
-
Komagataella pastoris
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.3.13
ethanol + O2
-
484910
Ogataea angusta
acetaldehyde + H2O2
-
-
-
?
1.1.3.13
ethanol + O2
82% of the activity with methanol
484910
Komagataella pastoris
acetaldehyde + H2O2
-
-
-
?
1.1.3.13
ethanol + O2
82% of the activity with methanol
484910
Komagataella pastoris IFP 206
acetaldehyde + H2O2
-
-
-
?
1.1.3.13
isobutanol + O2
1.2% of the activity with methanol
484910
Komagataella pastoris
isobutyraldehyde + H2O2
-
-
-
?
1.1.3.13
methanol + O2
-
484910
Komagataella pastoris
formaldehyde + H2O2
-
484910
Komagataella pastoris
?
1.1.3.13
methanol + O2
-
484910
Ogataea angusta
formaldehyde + H2O2
-
484910
Ogataea angusta
?
1.1.3.13
methanol + O2
key enzyme of methanol metabolism
484910
Komagataella pastoris
formaldehyde + H2O2
-
-
-
?
1.1.3.13
methanol + O2
key enzyme of methanol metabolism
484910
Ogataea angusta
formaldehyde + H2O2
-
-
-
?
1.1.3.13
methanol + O2
-
484910
Komagataella pastoris IFP 206
formaldehyde + H2O2
-
484910
Komagataella pastoris IFP 206
?
1.1.3.13
methanol + O2
key enzyme of methanol metabolism
484910
Komagataella pastoris IFP 206
formaldehyde + H2O2
-
-
-
?
1.1.3.13
n-butanol + O2
-
484910
Ogataea angusta
n-butanal + H2O2
-
-
-
?
1.1.3.13
n-butanol + O2
20% of the activity with methanol
484910
Komagataella pastoris
n-butanal + H2O2
-
-
-
?
1.1.3.13
n-propanol + O2
-
484910
Ogataea angusta
n-propanal + H2O2
-
-
-
?
1.1.3.13
n-propanol + O2
43% of the activity with methanol
484910
Komagataella pastoris
n-propanal + H2O2
-
-
-
?
1.1.3.13
n-propanol + O2
43% of the activity with methanol
484910
Komagataella pastoris IFP 206
n-propanal + H2O2
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.3.13
octamer
8 * 80000, SDS-PAGE
Komagataella pastoris
1.1.3.13
octamer
-
Ogataea angusta
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.3.13
37
-
-
Komagataella pastoris
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.1.3.13
18
45
half-maximal activity at 18°C and 45°C
Komagataella pastoris
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.3.13
7.5
-
-
Komagataella pastoris
1.1.3.13
8.5
-
-
Ogataea angusta
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.3.13
6.5
8.3
about half-maximal activity at pH 6.5 and 8.3
Komagataella pastoris
1.1.3.13
6.7
9.8
about half-maximal activity at pH 6.7 and 9.8
Ogataea angusta
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.1.3.13
7
8
stable
Komagataella pastoris
1.1.3.13
8
10
stable
Ogataea angusta
1.1.3.13
9
-
and above, inactivation
Komagataella pastoris