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Literature summary extracted from

  • Leach, F.R.; Webster, J.J.
    Commercially available firefly luciferase reagents (1986), Methods Enzymol., 133, 51-70.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.12.7 Luciferin preparations supplemented with extra luciferin provide maximum light emission Photinus pyralis

General Stability

EC Number General Stability Organism
1.13.12.7 bovine serum albumin stabilizes Photinus pyralis
1.13.12.7 EDTA, 2-mercaptoethanol, dithiothreitol and bovine serum albumin used as stabilizers Photinus pyralis
1.13.12.7 more stable in phosphate buffer than in Tricine buffer Photinus pyralis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.12.7 arsenate reversible inhibition Photinus pyralis
1.13.12.7 bovine serum albumin inhibition when present in large excess Photinus pyralis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.12.7 Mg2+
-
 Photinus pyralis

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.7 Photinus pyralis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.7 Photinus luciferin + O2 + ATP
-
 Photinus pyralis oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.12.7 25
-
-
 Photinus pyralis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.12.7 additional information
-
 higher stability in frozen state than in refrigerated Photinus pyralis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.12.7 7.8
-
 phosphate buffer Photinus pyralis