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Literature summary extracted from

  • Tsou, A.Y.; Ransom, S.C.; Gerlt, J.A.; Buechter, D.D.; Babbitt, P.C.; Kenyon, G.L.
    Mandelate pathway of Pseudomonas putida: sequence relationships involving mandelate racemase, (S)-mandelate dehydrogenase, and benzoylformate decarboxylase and expression of benzoylformate decarboxylase in Escherichia coli (1990), Biochemistry, 29, 9856-9862.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.7
-
Pseudomonas putida
1.2.1.28
-
Pseudomonas putida
4.1.1.7
-
Pseudomonas putida
5.1.2.2 expression in Escherichia coli Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.7 benzaldehyde + NADP+ + H2O Pseudomonas putida
-
benzoate + NADPH
-
?
1.2.1.28 benzaldehyde + NAD+ + H2O Pseudomonas putida
-
benzoate + NADH + H+
-
?
5.1.2.2 D-mandelate Pseudomonas putida first enzyme of pathway of oxidation of D,L-mandelate that permits certain strains of Pseudomonas putida to use mandelate as a sole source of carbon and energy L-mandelate
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.7 Pseudomonas putida
-
-
-
1.2.1.28 Pseudomonas putida
-
-
-
4.1.1.7 Pseudomonas putida
-
-
-
5.1.2.2 Pseudomonas putida
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.7 benzaldehyde + NADP+ + H2O
-
Pseudomonas putida benzoate + NADPH
-
?
1.2.1.28 benzaldehyde + NAD+ + H2O
-
Pseudomonas putida benzoate + NADH + H+
-
?
5.1.2.2 D-mandelate
-
Pseudomonas putida L-mandelate
-
?
5.1.2.2 D-mandelate first enzyme of pathway of oxidation of D,L-mandelate that permits certain strains of Pseudomonas putida to use mandelate as a sole source of carbon and energy Pseudomonas putida L-mandelate
-
?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.7 additional information
-
heat-labile Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.7 NADP+
-
Pseudomonas putida
1.2.1.28 NAD+
-
Pseudomonas putida