EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.1.1.65 | S254C | S254C and S254T are posttranslationally processed and active enzyme is made in vivo although in significantly reduced amounts. From the S254A mutant only the proenzyme form is made, which has no enzymatic activity. In the case of the S254C and S254T mutant proteins about 10-20% of the proenzyme is processed to the alpha and beta subunits, resulting in 15% and 2%, respectively of the level of activity of the wild-type enzyme | Escherichia coli |
4.1.1.65 | S254T | S254C and S254T are posttranslationally processed and active enzyme is made in vivo although in significantly reduced amounts. From the S254A mutant only the proenzyme form is made, which has no enzymatic activity. In the case of the S254C and S254T mutant proteins about 10-20% of the proenzyme is processed to the alpha and beta subunits, resulting in 15% and 2%, respectively of the level of activity of the wild-type enzyme | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.65 | Hydrazines | in presence of an amine | Escherichia coli | |
4.1.1.65 | hydroxylamine | in presence of an amine | Escherichia coli | |
4.1.1.65 | NaBH4 | in presence of an amine | Escherichia coli | |
4.1.1.65 | NaCNBH3 | in presence of an amine | Escherichia coli |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
4.1.1.65 | membrane | associated with cytoplasmic membrane | Escherichia coli | 16020 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.1.65 | 7332 | - |
x * 7332, alpha, + x * 28579, beta, the heterodimeric enzyme appears to form a defined multimeric structure in the absence of detergent and associate with detergent micelles to form a large detergent micelle-protein complex which is still made up of some multiple of the heterodimer | Escherichia coli |
4.1.1.65 | 28579 | - |
x * 7332, alpha, + x * 28579, beta, the heterodimeric enzyme appears to form a defined multimeric structure in the absence of detergent and associate with detergent micelles to form a large detergent micelle-protein complex which is still made up of some multiple of the heterodimer | Escherichia coli |
4.1.1.65 | 170000 | - |
sucrose density gradient centrifugation in absence of Triton | Escherichia coli |
4.1.1.65 | 200000 | - |
gel filtration in presence of Triton X-100 | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.1.65 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.1.65 | - |
Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.1.1.65 | 55 | - |
- |
Escherichia coli |
EC Number | Storage Stability | Organism |
---|---|---|
4.1.1.65 | -80°C to -20°C, stable for several years | Escherichia coli |
4.1.1.65 | 0°C, stable for several months | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.65 | Phosphatidyl-L-serine | - |
Escherichia coli | Phosphatidylethanolamine + CO2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.1.65 | dimer | x * 7332, alpha, + x * 28579, beta, the heterodimeric enzyme appears to form a defined multimeric structure in the absence of detergent and associate with detergent micelles to form a large detergent micelle-protein complex which is still made up of some multiple of the heterodimer | Escherichia coli |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.1.65 | 65 | - |
10 min, 50% loss of activity, membrane-associated enzyme | Escherichia coli |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
4.1.1.65 | 6.5 | 7.5 | maximal stability in the range | Escherichia coli |