Literature summary extracted from

Yoshida, H.; Tani, Y.; Yamada, H.
Isolation and identification of a pyrogallol producing bacterium from soil (1982), Agric. Biol. Chem., 46, 2539-2546.

No PubMed abstract available

General Stability

EC Number	General Stability	Organism
4.1.1.59	crude enzyme is very unstable, several attempts to purify the enzyme have so far been unsuccessful	Citrobacter sp.
4.1.1.59	dilution and dialysis completely inactivates	Citrobacter sp.

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
4.1.1.59	3.3	-	3,4,5-Trihydroxybenzoate	-	Citrobacter sp.

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
4.1.1.59	3,4,5-Trihydroxybenzoate	Citrobacter sp.	inducible by protocatechuic acid, 3,5-dihydroxybenzoate, and 3-hydroxybenzoate	?	-	?
4.1.1.59	3,4,5-Trihydroxybenzoate	Citrobacter sp. 64-1	inducible by protocatechuic acid, 3,5-dihydroxybenzoate, and 3-hydroxybenzoate	?	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
4.1.1.59	Citrobacter sp.	-	-	-
4.1.1.59	Citrobacter sp. 64-1	-	-	-

Storage Stability

EC Number	Storage Stability	Organism
4.1.1.59	5°C, 90% loss of activity after 2 days, crude enzyme	Citrobacter sp.

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4.1.1.59	3,4,5-Trihydroxybenzoate	-	Citrobacter sp.	Pyrogallol + CO2	-	?
4.1.1.59	3,4,5-Trihydroxybenzoate	-	Citrobacter sp. 64-1	Pyrogallol + CO2	-	?
4.1.1.59	3,4,5-Trihydroxybenzoate	inducible by protocatechuic acid, 3,5-dihydroxybenzoate, and 3-hydroxybenzoate	Citrobacter sp.	?	-	?
4.1.1.59	3,4,5-Trihydroxybenzoate	inducible by protocatechuic acid, 3,5-dihydroxybenzoate, and 3-hydroxybenzoate	Citrobacter sp. 64-1	?	-	?

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4.1.1.59	6	-	-	Citrobacter sp.

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)