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Literature summary extracted from

  • Pujar, B.G.; Ribbons, D.W.
    Phthalate metabolism in Pseudomonas fluorescens PHK: purification and properties of 4,5-dihydroxyphthalate decarboxylase (1985), Appl. Environ. Microbiol., 49, 374-376.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.55 1,10-phenanthroline
-
 Pseudomonas fluorescens
4.1.1.55 5,5'-dithiobis(2-nitrobenzoate)
-
 Pseudomonas fluorescens
4.1.1.55 Cd2+
-
 Pseudomonas fluorescens
4.1.1.55 Cu2+
-
 Pseudomonas fluorescens
4.1.1.55 Fe2+
-
 Pseudomonas fluorescens
4.1.1.55 NEM
-
 Pseudomonas fluorescens
4.1.1.55 PCMB
-
 Pseudomonas fluorescens
4.1.1.55 Zn2+
-
 Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.55 0.0104
-
 4,5-Dihydroxyphthalate
-
 Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.55 66000
-
 6 * 66000, SDS-PAGE Pseudomonas fluorescens
4.1.1.55 420000
-
 gel filtration Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.55 4,5-Dihydroxyphthalate Pseudomonas fluorescens enzyme is involved in o-phthalate catabolism ?
-
 ?
4.1.1.55 4,5-Dihydroxyphthalate Pseudomonas fluorescens PHK enzyme is involved in o-phthalate catabolism ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.55 Pseudomonas fluorescens
-
-
-
4.1.1.55 Pseudomonas fluorescens PHK
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.55
-
 Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.55 4.8
-
-
 Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.55 4,5-Dihydroxyphthalate
-
 Pseudomonas fluorescens 3,4-Dihydroxybenzoate + CO2
-
 ?
4.1.1.55 4,5-Dihydroxyphthalate
-
 Pseudomonas fluorescens PHK 3,4-Dihydroxybenzoate + CO2
-
 ?
4.1.1.55 4,5-Dihydroxyphthalate enzyme is involved in o-phthalate catabolism Pseudomonas fluorescens ?
-
 ?
4.1.1.55 4,5-Dihydroxyphthalate enzyme is involved in o-phthalate catabolism Pseudomonas fluorescens PHK ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.55 hexamer 6 * 66000, SDS-PAGE Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.55 6.8
-
-
 Pseudomonas fluorescens