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Literature summary extracted from

  • Igarashi, K.; Eguchi, K.; Tanaka, M.; Hirose, S.
    Effect of polyamines on isoleucyl-tRNA formation by rat liver isoleucyl-tRNA synthetase (1978), Eur. J. Biochem., 82, 301-307.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.1.1.5 spermidine in presence of 50 mM K+, addition of spermine prevents inhibitory effect of 1.5 mM Mg2+ or above. In presence of 200 mM K+, the addition of spermine stimulates isoleucyl-tRNA formation in presence of Mg2+, 0-5 mM Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.5 Mg2+ in presence of 50 mM K+ and in absence of polyamines, the optimal Mg2+ concentration for Ile-tRNA formation is 1 mM, an increase in Mg2+ concentration markedly inhibits Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.5 Mg2+ in presence of 50 mM K+ and in absence of polyamines, the optimal Mg2+ concentration for Ile-tRNA formation is 1 mM, an increase in Mg2+ concentration markedly inhibits Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.5 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.5
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.1.1.5 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.5 0.00084
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.5 ATP + L-isoleucine + tRNAIle
-
 Rattus norvegicus AMP + diphosphate + L-isoleucyl-tRNAIle
-
 ?