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Literature summary extracted from

  • Yamanoha, B.; Cohen, S.S.
    S-Adenosylmethionine decarboxylase and spermidine synthase from Chineses cabbage (1985), Plant Physiol., 78, 784-790.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.50 putrescine no stimulation Brassica rapa subsp. pekinensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.50 (5-deoxy-5-adenosyl)(3-aminopropyl)methylsulfonium salt competitive with respect to S-adenosylmethionine; i.e. decarboxylated S-adenosylmethionine Brassica rapa subsp. pekinensis
4.1.1.50 methylglyoxal bis(guanylhydrazone) strong Brassica rapa subsp. pekinensis
4.1.1.50 putrescine
-
 Brassica rapa subsp. pekinensis
4.1.1.50 spermidine
-
 Brassica rapa subsp. pekinensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.50 0.038
-
 S-adenosyl-L-methionine
-
 Brassica rapa subsp. pekinensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.1.50 protoplast from leaves of growing healthy or virus-infected plants Brassica rapa subsp. pekinensis
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.50 Mg2+ no stimulaton Brassica rapa subsp. pekinensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.16 81000
-
 gel filtration Brassica rapa subsp. pekinensis

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.16 Brassica rapa subsp. pekinensis
-
 Chinese cabbage, var Pak Choy
-
4.1.1.50 Brassica rapa subsp. pekinensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.16 ammonium sulfate fractionation, affinity chromatography and gel filtration, 1900fold purified Brassica rapa subsp. pekinensis
4.1.1.50
-
 Brassica rapa subsp. pekinensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.16 leaf protoplasts from healthy or turnip yellow mosaic virus infected leaves Brassica rapa subsp. pekinensis
-
4.1.1.50 leaf of growing healthy or virus-infected plants Brassica rapa subsp. pekinensis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.16 1.07
-
-
 Brassica rapa subsp. pekinensis
4.1.1.50 additional information
-
-
 Brassica rapa subsp. pekinensis

Storage Stability

EC Number Storage Stability Organism
2.5.1.16 4ºC, unstable Brassica rapa subsp. pekinensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.50 S-adenosyl-L-methionine
-
 Brassica rapa subsp. pekinensis (5-deoxy-5-adenosyl)(3-aminopropyl)methylsulfonium salt + CO2
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.16 28
-
 assay at Brassica rapa subsp. pekinensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.50 7.3 7.4 phosphate buffer Brassica rapa subsp. pekinensis