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Literature summary extracted from

  • Asakawa, T.; Wada, H.; Yamano, T.
    Enzymatic conversion of phenylpyruvate to phenylacetate (1968), Biochim. Biophys. Acta, 170, 375-391.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.1.1.43 thiamine diphosphate stabilizes Acinetobacter sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.43 Cu2+
-
Acinetobacter sp.
4.1.1.43 PCMB
-
Acinetobacter sp.
4.1.1.43 Zn2+
-
Acinetobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.43 0.0051
-
phenylpyruvate
-
Acinetobacter sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.43 Mg2+ required Acinetobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.43 Acinetobacter sp.
-
other name: Achromobacter eurydice
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.43
-
Acinetobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.43 2-Oxo-gamma-ethylthiobutanoate at approximately 30% of the activity with phenylpyruvate Acinetobacter sp. beta-Ethiopropanal + CO2
-
?
4.1.1.43 2-Oxo-isohexanoate at approximately 5% of the activity with phenylpyruvate Acinetobacter sp. ?
-
?
4.1.1.43 2-Oxohexanoate at approximately 31% of the activity with phenylpyruvate Acinetobacter sp. Pentanal + CO2
-
?
4.1.1.43 4-methylthio-2-oxobutanoate at approximately 23% of the activity with phenylpyruvate Acinetobacter sp. beta-methiopropanal + CO2
-
?
4.1.1.43 Indolepyruvate at 48% of the activity with phenylpyruvate Acinetobacter sp. Indoleethanal + CO2
-
?
4.1.1.43 Phenylpyruvate
-
Acinetobacter sp. Phenylacetaldehyde + CO2
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.43 7.6
-
-
Acinetobacter sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.1.43 5.5
-
30°C, 1 h, about 60% loss of activity Acinetobacter sp.
4.1.1.43 6.3 6.4 30°C, 1 h, completely stable, maximal stability Acinetobacter sp.
4.1.1.43 8.8
-
30°C, 1 h, 80% loss of activity Acinetobacter sp.

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.43 thiamine diphosphate Km: 0.0013-0.0017 mM Acinetobacter sp.