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Literature summary extracted from

  • Bott, M.; Pfister, K.; Burda, P.; Kalbermatter, O.; Woehlke, G.; Dimroth, P.
    Methylmalonyl-CoA decarboxylase from Propionibacterium modestum. Cloning and sequencing of the structural genes and purification of the enzyme complex (1997), Eur. J. Biochem., 250, 590-599.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.2.4.3
-
 Propionigenium modestum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.2.4.3 0.012
-
 (S)-methylmalonyl-CoA
-
 Propionigenium modestum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.2.4.1 41200
-
 x * 41200, calculated Propionigenium modestum
7.2.4.3 13100
-
 x * 56100, alpha, carboxyltransferase, + x * 41200, beta, carboxybiotin-carrier-protein decarboxylase, + x * 13100, gamma, biotin carrier protein, + x * 14200, delta, essential for the assembly of the complex, calculation of the MW from nucleotide sequence Propionigenium modestum
7.2.4.3 14200
-
 x * 56100, alpha, carboxyltransferase, + x * 41200, beta, carboxybiotin-carrier-protein decarboxylase, + x * 13100, gamma, biotin carrier protein, + x * 14200, delta, essential for the assembly of the complex, calculation of the MW from nucleotide sequence Propionigenium modestum
7.2.4.3 17000
-
 x * 56000 + x * 35000 + x * 17500 + x * 17000, SDS-PAGE Propionigenium modestum
7.2.4.3 17500
-
 x * 56000 + x * 35000 + x * 17500 + x * 17000, SDS-PAGE Propionigenium modestum
7.2.4.3 35000
-
 x * 56000 + x * 35000 + x * 17500 + x * 17000, SDS-PAGE Propionigenium modestum
7.2.4.3 41200
-
 x * 56100, alpha, carboxyltransferase, + x * 41200, beta, carboxybiotin-carrier-protein decarboxylase, + x * 13100, gamma, biotin carrier protein, + x * 14200, delta, essential for the assembly of the complex, calculation of the MW from nucleotide sequence Propionigenium modestum
7.2.4.3 56000
-
 x * 56000 + x * 35000 + x * 17500 + x * 17000, SDS-PAGE Propionigenium modestum
7.2.4.3 56100
-
 x * 56100, alpha, carboxyltransferase, + x * 41200, beta, carboxybiotin-carrier-protein decarboxylase, + x * 13100, gamma, biotin carrier protein, + x * 14200, delta, essential for the assembly of the complex, calculation of the MW from nucleotide sequence Propionigenium modestum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.2.4.3 (S)-methylmalonyl-CoA + Na+[side 1] + H+[side 2] Propionigenium modestum the enzyme catalyzes the only energy-conserving step during succinate fermentation. The decarboxylation of (S)-methylmalonyl-CoA is coupled to the vectorial transport of Na+ across the cytoplasmic membrane, thereby creating a sodium ion motive force that is used for ATP synthesis propanoyl-CoA + CO2 + Na+[side 2]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.2.4.1 Propionigenium modestum
-
 carboxybiotin decarboxylase is part of the methylmalonyl-CoA decarboxylase
-
7.2.4.3 Propionigenium modestum
-
 DSM 2376
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.2.4.1 methymalonyl-CoA decarboxylase complex Propionigenium modestum
7.2.4.3
-
 Propionigenium modestum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.2.4.3 additional information
-
-
 Propionigenium modestum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.2.4.3 (S)-methylmalonyl-CoA + Na+[side 1] + H+[side 2]
-
 Propionigenium modestum propanoyl-CoA + CO2 + Na+[side 2]
-
 ?
7.2.4.3 (S)-methylmalonyl-CoA + Na+[side 1] + H+[side 2] the enzyme catalyzes the only energy-conserving step during succinate fermentation. The decarboxylation of (S)-methylmalonyl-CoA is coupled to the vectorial transport of Na+ across the cytoplasmic membrane, thereby creating a sodium ion motive force that is used for ATP synthesis Propionigenium modestum propanoyl-CoA + CO2 + Na+[side 2]
-
 ?

Subunits

EC Number Subunits Comment Organism
7.2.4.1 ? x * 41200, calculated Propionigenium modestum
7.2.4.3 ? x * 56100, alpha, carboxyltransferase, + x * 41200, beta, carboxybiotin-carrier-protein decarboxylase, + x * 13100, gamma, biotin carrier protein, + x * 14200, delta, essential for the assembly of the complex, calculation of the MW from nucleotide sequence Propionigenium modestum
7.2.4.3 ? x * 56000 + x * 35000 + x * 17500 + x * 17000, SDS-PAGE Propionigenium modestum

Synonyms

EC Number Synonyms Comment Organism
7.2.4.1 MmdB
-
 Propionigenium modestum