Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Tong, J.H.; D'Iorio, A.
    Solubilization and partial purification of particulate catechol-O-methyltransferase from rat liver (1977), Can. J. Biochem., 55, 1108-1113.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.6 beta-thujaplicin 70-100% inhibition at 0.2 mM Rattus norvegicus
2.1.1.6 Mg2+ required for activity Rattus norvegicus
2.1.1.6 N-ethylmaleimide 70-100% inhibition at 0.2 mM Rattus norvegicus
2.1.1.6 p-hydroxymercuribenzoate 70-100% inhibition at 0.2 mM Rattus norvegicus
2.1.1.6 pyrogallol 70-100% inhibition at 0.2 mM Rattus norvegicus
2.1.1.6 tropolone 70-100% inhibition at 0.2 mM Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.6 additional information
-
 additional information
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.6 microsome
-
 Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.6 Mg2+ required Rattus norvegicus
2.1.1.6 Mg2+ optimum range: 2-5 mM Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.6 21000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.6 additional information Rattus norvegicus membrane protein and (or) lipid components may play an important role in catecholamine metabolism ?
-
 ?
2.1.1.6 S-adenosyl-L-methionine + a catechol Rattus norvegicus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.6 Rattus norvegicus
-
 particulate enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.6 microsomal Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.6 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.6 additional information
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.6 additional information membrane protein and (or) lipid components may play an important role in catecholamine metabolism Rattus norvegicus ?
-
 ?
2.1.1.6 S-adenosyl-L-methionine + a catechol
-
 Rattus norvegicus ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.6 8 8.5
-
 Rattus norvegicus